Cloning and expression of a cDNA encoding a mouse brain orphanin FQ/nociceptin precursor.
Cloning and expression of a cDNA encoding a mouse brain orphanin FQ/nociceptin precursor.
复制标题
编码小鼠脑孤啡肽 FQ/伤害感受素前体的 cDNA 的克隆和表达。
DOI：
10.1042/bj3150011
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Pasternak,GW
中科院分区：
文献类型：
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作者：
Pan,YX;Xu,J;Pasternak,GW
通过使用逆转录-PCR方法，我们从小鼠脑中克隆了一个肽前体，其包含FQ/伤害感受素的序列。小鼠FQ/痛敏肽的序列在氨基酸水平上与从大鼠和猪脑中分离的序列相同。对编码前体的mRNA的北方分析揭示了一条约为100 bp的单一条带。1 kb，在脑中水平最高，在肾和脾中水平低得多。Southern分析与单个基因一致。小鼠的前体肽含有两个其他的推定肽。FQ/伤害感受素的上游是一个41个氨基酸的肽，除了6个氨基酸的插入外，它与从大鼠序列预测的相应的35个氨基酸的肽几乎相同。有趣的是，小鼠在该肽中含有三重AEPGAD重复序列，这在大鼠序列中未观察到。在FQ/痛敏肽序列的下游是另一个17个氨基酸的肽，其与在大鼠中发现的肽相同。
By using a reverse transcription–PCR approach we have cloned a peptide precursor from mouse brain which contains the sequence of orphanin FQ/nociceptin. The mouse sequence of orphanin FQ/nociceptin is identical at the amino acid level with that isolated from rat and porcine brain. Northern analysis of the mRNA encoding the precursor reveals a single band of approx. 1 kb, with the highest levels in the brain and much lower levels in kidney and spleen. Southern analysis is consistent with a single gene. The precursor peptide from mouse contains two other putative peptides. Upstream from the orphanin FQ/nociceptin is a 41-amino-acid peptide which is almost identical, except for a six-amino-acid insertion, with the corresponding 35-amino-acid peptide predicted from the rat sequence. Interestingly, the mouse contains a triple AEPGAD repeat within this peptide that is not seen in the rat sequence. Downstream from the orphanin FQ/nociceptin sequence is another 17-amino-acid peptide which is identical with that found in the rat.