Expression of messenger RNA for keratinocyte growth factor in human cholesteatoma.
Expression of messenger RNA for keratinocyte growth factor in human cholesteatoma.
复制标题
人胆脂瘤中角化细胞生长因子信使 RNA 的表达。
DOI：
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发表时间：
1996
期刊：
影响因子：
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通讯作者：
Hiroshi Moriyama
中科院分区：
文献类型：
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作者：
Hiromi Kojima;Akio Matsuhisa;M. Shiwa;Yosuke Kamide;Mariko Nakamura;Tsuneya Ohno;Hiroshi Moriyama
目的
了解角化细胞生长因子在中耳炎上皮和上皮下结缔组织间的旁分泌调节作用。
设计
人胎儿成纤维细胞特异性DNA探针的制备及非放射性原位杂交法检测角化细胞生长因子mRNA在中耳乳突瘤上皮下肉芽组织中的定位
参与者
手术切除12例中耳乳突瘤标本。从6例患者的外耳道收集的正常皮肤标本作为对照。
结果
角质形成细胞生长因子信使RNA的特异性信号在外耳道的正常皮肤中不表达，但在中耳炎标本的上皮下结缔组织成纤维细胞中观察到。仅在从上皮下结缔组织厚且增殖且炎症强烈的患者采集的标本中观察到信号。
结论
角化细胞生长因子参与的旁分泌调节机制可能是中耳炎上皮细胞增殖的重要机制。上皮下结缔组织在中耳炎的发生、发展过程中起重要作用，尤其是在炎症条件下。
OBJECTIVE
To understand the interaction between cholesteatoma epithelium and subepithelial connective tissue as a paracrine regulation by keratinocyte growth factor.
DESIGN
Preparation of a specific DNA probe from human fetal fibroblast and detection of localization of keratinocyte growth factor messenger RNA in subepithelial granulation tissue of middle-ear cholesteatoma by a nonradioactive in situ hybridization method.
PARTICIPANTS
The cholesteatoma specimens were excised from 12 patients during surgery. Normal skin specimens collected from the external ear canal of six patients were used as controls.
RESULTS
Signals specific for keratinocyte growth factor messenger RNA were not expressed in the normal skin of the external ear canal, but were observed in fibroblasts of subepithelial connective tissue of cholesteatoma specimens. Signals were observed only in specimens collected from patients whose subepithelial connective tissue was thick and proliferated and whose inflammation was strong.
CONCLUSIONS
A paracrine regulation mechanism involving keratinocyte growth factor may exist for proliferation of epithelium of cholesteatoma. The subepithelial connective tissue of cholesteatoma may play an important role in the proliferation and development of the cholesteatoma, especially under inflammatory conditions.