Survival and transfer ability of phylogenetically diverse bacterial endosymbionts in environmental Acanthamoeba isolates
Survival and transfer ability of phylogenetically diverse bacterial endosymbionts in environmental Acanthamoeba isolates
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DOI：
10.1111/j.1758-2229.2009.00094.x
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发表时间：
2010-08-01
影响因子：
3.3
通讯作者：
Yamaguchi, Hiroyuki
中科院分区：
文献类型：
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作者：
Matsuo, Junji;Kawaguchi, Kouhei;Yamaguchi, Hiroyuki
专性胞内细菌通常被发现作为棘阿米巴的内共生体;然而，它们在阿米巴中的存活和转移到阿米巴的能力目前还没有特征。在这项研究中，六个细菌内共生体，发现在五个环境阿米巴分离株（S13，R18，S23，S31，S40）从不同地点的札幌市，日本，进行了表征。系统发育分析表明，3个细菌内共生体（eS 23，eS 31，eS 40 a）属于α-和β-变形菌门，其余内共生体（eS 13，eR 18，eS 40 b）属于衣原体目。阿米巴分离株（S40）含有两种遗传学上不同的细菌内共生体（eS 40 a、eS 40 b）。荧光原位杂交分析表明，所有的细菌内共生体广泛定位在阿米巴。透射电子显微镜也显示内共生体为杆状（eS 23、eS 31、eS 40 a）或球形或新月形（eS 13、eR 18、eS 40 b）。使用常规培养方法没有成功培养这些细菌，但通过活/死染色和RT-PCR方法证实了内共生体的活力。然而，来源于原始宿主细胞的内共生体（eR 18除外）失去了转移到另一种阿米巴菌株[ATCC菌株（C3），环境菌株（S14，R23，S24）]的能力。因此，我们的数据表明，阿米巴中发现的遗传多样性细菌内共生体与阿米巴保持稳定的相互作用，但可转移性是有限的。
P>Obligate intracellular bacteria are commonly found as endosymbionts of acanthamoebae; however, their survival in and ability to transfer to amoebae are currently uncharacterized. In this study, six bacterial endosymbionts, found in five environmental Acanthamoeba isolates (S13, R18, S23, S31, S40) from different locations of Sapporo city, Japan, were characterized. Phylogenetic analysis revealed that three bacterial endosymbionts (eS23, eS31, eS40a) belonged to alpha- and beta-Proteobacteria phyla and the remaining endosymbionts (eS13, eR18, eS40b) belonged to the order Chlamydiales. The Acanthamoeba isolate (S40) contained two phylogenetically different bacterial endosymbionts (eS40a, eS40b). Fluorescent in situ hybridization analysis showed that all bacterial endosymbionts were diffusely localized within amoebae. Transmission electron microscopy also showed that the endosymbionts were rod-shaped (eS23, eS31, eS40a) or sphere- or crescent-shaped (eS13, eR18, eS40b). No successful culture of these bacteria was achieved using conventional culture methods, but the viability of endosymbionts was confirmed by live/dead staining and RT-PCR methods. However, endosymbionts (except eR18) derived from original host cells lost the ability to be transferred to another Acanthamoebae strains [ATCC strain (C3), environmental strains (S14, R23, S24)]. Thus, our data demonstrate that phylogenetically diverse bacterial endosymbionts found in amoebae maintain a stable interaction with amoebae, but the transferability is limited.