Transcriptomic Signatures of Tacaribe Virus-Infected Jamaican Fruit Bats.
Transcriptomic Signatures of Tacaribe Virus-Infected Jamaican Fruit Bats.
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DOI：
10.1128/msphere.00245-17
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发表时间：
2017-09-01
期刊：
影响因子：
4.8
通讯作者：
Frietze, Seth
中科院分区：
文献类型：
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作者：
Gerrard, Diana L;Hawkinson, Ann;Frietze, Seth
塔卡里贝病毒(Tacaribe Virus，TCRV)是20世纪50年代首次从蝙蝠体内分离到的一种哺乳动物虫媒病毒。随后对牙买加果蝠(Arbeus Jamaicensis)的实验感染引起了一种类似于自然感染蝙蝠的疾病。尽管蝙蝠是导致人类疾病的病毒的主要宿主，但人们对蝙蝠与病原体之间的相互作用知之甚少。我们进行了一项转录组范围的研究，以阐明实验感染TCRV的牙买加果蝠的反应。对多个组织的差异基因表达分析揭示了与先天抗病毒反应相关的全局和器官特异性反应，包括干扰素α/β和Toll样受体信号、补体激活级联反应和细胞因子信号等。与适应性免疫反应有关的编码蛋白的基因也发生了变化，如伽马干扰素信号和CD28家族对T细胞的共刺激作用。免疫球蛋白基因在感染蝙蝠的脾组织中的表达也升高，包括免疫球蛋白、免疫球蛋白A和免疫球蛋白E亚型。这些结果表明，对TCRV感染发生了主动的先天和获得性免疫反应，但不能预防致命的疾病。这种从头组装提供了牙买加果蝠及其宿主对TCRV感染反应的高通量数据集，这仍然是了解蝙蝠抗病毒反应所涉及的分子签名的宝贵工具。作为与人类疾病相关的病毒的宿主，蝙蝠很重要，但人们对蝙蝠和病毒之间的相互作用知之甚少。以感染塔卡贝病毒(Tacaribe Virus，TCRV)的牙买加果蝠为模型，研究了不同组织中基因表达对感染的反应，并确定了参与感染反应的途径。这份报告是迄今为止该物种中最详细的基因发现工作，也是第一次描述蝙蝠在致病病毒感染期间的免疫基因表达反应。
Tacaribe virus (TCRV) is a mammalian arenavirus that was first isolated from artibeus bats in the 1950s. Subsequent experimental infection of Jamaican fruit bats (Artibeus jamaicensis) caused a disease similar to that of naturally infected bats. Although substantial attention has focused on bats as reservoir hosts of viruses that cause human disease, little is known about the interactions between bats and their pathogens. We performed a transcriptome-wide study to illuminate the response of Jamaican fruit bats experimentally infected with TCRV. Differential gene expression analysis of multiple tissues revealed global and organ-specific responses associated with innate antiviral responses, including interferon alpha/beta and Toll-like receptor signaling, activation of complement cascades, and cytokine signaling, among others. Genes encoding proteins involved in adaptive immune responses, such as gamma interferon signaling and costimulation of T cells by the CD28 family, were also altered in response to TCRV infection. Immunoglobulin gene expression was also elevated in the spleens of infected bats, including IgG, IgA, and IgE isotypes. These results indicate an active innate and adaptive immune response to TCRV infection occurred but did not prevent fatal disease. This de novo assembly provides a high-throughput data set of the Jamaican fruit bat and its host response to TCRV infection, which remains a valuable tool to understand the molecular signatures involved in antiviral responses in bats. IMPORTANCE As reservoir hosts of viruses associated with human disease, little is known about the interactions between bats and viruses. Using Jamaican fruit bats infected with Tacaribe virus (TCRV) as a model, we characterized the gene expression responses to infection in different tissues and identified pathways involved with the response to infection. This report is the most detailed gene discovery work in the species to date and the first to describe immune gene expression responses in bats during a pathogenic viral infection.