Lipoprotein lipase bound to apolipoprotein B lipoproteins accelerates clearance of postprandial lipoproteins in humans.
Lipoprotein lipase bound to apolipoprotein B lipoproteins accelerates clearance of postprandial lipoproteins in humans.
复制标题
与载脂蛋白 B 脂蛋白结合的脂蛋白脂肪酶可加速人体餐后脂蛋白的清除。
DOI：
10.1161/01.atv.0000203512.01007.3d
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Sacks,FrankM
中科院分区：
文献类型：
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作者：
Zheng,Chunyu;Murdoch,SusanJ;Brunzell,JohnD;Sacks,FrankM
目的-细胞和动物模型实验表明，脂蛋白脂酶（LpL）与载脂蛋白（apo）B脂蛋白结合，通过受体介导的途径增强其摄取。这是未知的，这条途径是否是重要的human.Methods和Results-ApoB脂蛋白与LpL分离正常受试者口服脂肪负荷后，通过免疫亲和层析，并进一步分离成apoB 100和apoB 48脂蛋白。餐后，与LpL相比，apoB脂蛋白与LpL相比有显著更大的增加（4- 10倍）和更快的清除率（5- 8倍）。无论是否还含有apoE，具有LpL的apoB脂蛋白的清除率均增强。LpL对于apoB 48脂蛋白的清除特别重要，其中25%（范围，11%至31%）可以在餐后状态期间与LpL一起从循环中去除。与无LpL的脂蛋白相比，具有LpL的载脂蛋白B的尺寸更大，并且富含甘油三酯、胆固醇和载脂蛋白E。然而，无论是大小，也不载脂蛋白E的内容解释了更快的清除率的LPL-含lipoprotein. Conclusion血浆LPL可以像载脂蛋白，以提高载脂蛋白B脂蛋白在人类的清除，一种机制，特别是重要的肠道脂蛋白在餐后状态。
Objectives—Experiments in cells and animal models show that lipoprotein lipase (LpL) bound to apolipoprotein (apo)B lipoproteins enhances their uptake by receptor mediated pathways. It is unknown whether this pathway is important in humans.Methods and Results—ApoB lipoproteins with LpL were isolated from normal subjects after oral fat loading by immunoaffinity chromatography and were further separated into apoB100 and apoB48 lipoproteins. Postprandially, apoB lipoproteins with LpL had significantly greater increases (4- to 10-fold) and faster rates of clearance (5- to 8-fold) percentage-wise than those without LpL. apoB lipoproteins with LpL had enhanced clearance regardless of whether they also contained apoE. LpL was particularly important for the clearance of apoB48 lipoproteins, of which 25% (range, 11% to 31%) could be removed from circulation together with LpL during the postprandial state. apoB lipoproteins with LpL were larger in size and were enriched in triglyceride, cholesterol, and apoE compared with those without LpL. However, neither size nor apoE content explained the faster clearance rates of LpL-containing lipoproteins.Conclusion—Plasma LpL may act like an apolipoprotein to enhance the clearance of apoB lipoproteins in humans, a mechanism particularly important for intestinal lipoproteins in the postprandial state.