Rapid in vitro propagation of medicinally important Aquilaria agallocha.
Rapid in vitro propagation of medicinally important Aquilaria agallocha.
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DOI：
10.1631/jzus.2005.b0849
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发表时间：
2005-08-01
期刊：
影响因子：
--
通讯作者：
Hu, Lan-juan
中科院分区：
文献类型：
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作者：
He, Meng-ling;Qi, Shu-yuan;Hu, Lan-juan
沉香可以生产香沉香用于熏香，传统医药和其他产品。以沉香实生苗为外植体，通过器官发生途径建立了一套高效的植株再生体系。在MS +1.3 μ mol/L BA（6-苄基氨基嘌呤）培养基上，前7周芽大量产生，后7周芽在MS +1.3 μ mol/L BA+0.5 μ mol/L NAA（萘乙酸）培养基上伸长，14周内每个外植体产生2.3个2cm长的芽。试管苗在1/2 MS培养基上用5 μ mol/LNAA浸泡48 h后生根，两周后生根率达96.7%。所有驯化后存活的植株在盆中生长良好。
Aquilaria agallocha can produce fragrant agarwood used for incense, traditional medicine and other products. An efficient plant regeneration system was established via organogenesis from shoots developed from seedlings of Aquilaria agallocha. Shoots generated many buds on MS medium supplemented with 1.3 micromol/L BA (6-benzylaminopurine) in the first 7 weeks, and the buds elongated on MS medium with 1.3 micromol/L BA+0.5 micromol/L NAA (naphthaleneacetic acid) in another 7 weeks, 2.3 shoots 2 cm in length per explant were obtained within 14 weeks. Plantlets were rooted on 1/2 MS medium after being immersed in 5 micromol/L NAA for 48 h, 96.7% of the roots grew up two weeks later. All plantlets that survived acclimatization grew well in the pots.