Patterning on Topography for Generation of Cell Culture Substrates with Independent Nanoscale Control of Chemical and Topographical Extracellular Matrix Cues.
Patterning on Topography for Generation of Cell Culture Substrates with Independent Nanoscale Control of Chemical and Topographical Extracellular Matrix Cues.
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DOI：
10.1002/cpcb.25
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发表时间：
2017-06-19
影响因子：
--
通讯作者：
Feinberg AW
中科院分区：
文献类型：
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作者：
Sevcik EN;Szymanski JM;Jallerat Q;Feinberg AW
细胞微环境在许多生物学过程中起着重要作用，包括发育和疾病进展。其关键是细胞外基质（ECM），一种复杂的生物聚合物网络，作为物理，化学和机械提示的主要不溶性信号网络。在体外，在微米和纳米尺度上设计ECM的能力是系统地询问ECM特性对细胞反应的影响的关键工具。具体而言，形貌和化学表面图案化已被证明可以指导生物材料表面上的细胞排列和组织结构，然而，已经证明独立控制这些表面特性具有挑战性。该方案描述了一种称为拓扑图案化（PoT）的方法，将ECM蛋白质的2D纳米粒子设计到拓扑复杂的基底上，这使得能够独立控制物理和化学表面特性。应用包括询问基本的细胞表面相互作用和工程接口，可以直接细胞和/或组织功能。
The cell microenvironment plays an important role in many biological processes, including development and disease progression. Key to this is the extracellular matrix (ECM), a complex biopolymer network serving as the primary insoluble signaling network for physical, chemical, and mechanical cues. In vitro, the ability to engineer the ECM at the micro- and nanoscales is a critical tool to systematically interrogate the influence of ECM properties on cellular responses. Specifically, both topographical and chemical surface patterning has been shown to direct cell alignment and tissue architecture on biomaterial surfaces, however, it has proven challenging to independently control these surface properties. This protocol describes a method termed Patterning on Topography (PoT) to engineer 2D nanopatterns of ECM proteins onto topographically complex substrates, which enables independent control of physical and chemical surface properties. Applications include interrogation of fundamental cell-surface interactions and engineering interfaces that can direct cell and/or tissue function.