Development of handheld induction heaters for magnetic fluid hyperthermia applications andin-vitroevaluation on ovarian and prostate cancer cell lines.
Development of handheld induction heaters for magnetic fluid hyperthermia applications andin-vitroevaluation on ovarian and prostate cancer cell lines.
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DOI：
10.1088/2057-1976/acbeaf
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发表时间：
2023-03-10
影响因子：
1.4
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中科院分区：
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目的：磁流体热疗（MFH）是一种在肿瘤治疗中具有潜在应用前景的实验性技术。该方法旨在通过外部施加的交变磁场（AMF）激发磁性纳米颗粒，使肿瘤部位达到约41 °C-47 °C，预计会发生细胞死亡。应用具有高空间分辨率的AMF仍然是一个挑战。来自当前和预期MFH施源器的AMF覆盖相对较大的区域;不适合在治疗区域附近具有金属植入物的患者。因此，临床上需要更小的磁场施加器。为此，开发了腹腔镜感应加热器（LIH）和经直肠感应加热器（TRIH）。方法：将微型“煎饼”线圈缠绕并插入3D打印的外壳中。卵巢癌（SKOV-3，A2780）和前列腺癌（PC-3，LNCaP）细胞系用于评估仪器在体外杀死癌细胞的能力，使用Synomag®-D纳米颗粒作为热介质。NIH 3 T3正常细胞系也与两种器械一起使用，以观察这些细胞是否耐受所应用的条件。结果：LIH和TRIH达到的磁场强度分别为42.6 kA m−1（326 kHz）和26.3 kA m−1（303 kHz）。样品中达到的温度为LIH的41 °C和TRIH的43 °C。这两种仪器都成功地杀死了癌细胞，对正常细胞的影响最小。结论：这项工作提出了第一条手持式医疗感应加热器，并有可能成为现有癌症治疗的补充。重要性：这些仪器可以使MFH模式的发展，这将有助于这种热治疗的临床翻译。
Objective: Magnetic fluid hyperthermia (MFH) is a still experimental technique found to have a potential application in the treatment of cancer. The method aims to reach around 41 °C–47 °C in the tumor site by exciting magnetic nanoparticles with an externally applied alternating magnetic field (AMF), where cell death is expected to occur. Applying AMFs with high spatial resolution is still a challenge. The AMFs from current and prospective MFH applicators cover relatively large areas; being not suitable for patients having metallic implants near the treatment area. Thus, there will be a clinical need for smaller magnetic field applicators. To this end, a laparoscopic induction heater (LIH) and a transrectal induction heater (TRIH) were developed. Methods: Miniature ‘pancake’ coils were wound and inserted into 3D printed enclosures. Ovarian (SKOV-3, A2780) and prostate (PC-3, LNCaP) cancer cell lines were used to evaluate the instruments’ capabilities in killing cancer cells in vitro, using Synomag®-D nanoparticles as the heat mediators. NIH3T3 normal cell lines were also used with both devices to observe if these cells tolerated the conditions applied. Results: Magnetic field intensities reached by the LIH and TRIH were 42.6 kA m−1 at 326 kHz and 26.3 kA m−1 at 303 kHz, respectively. Temperatures reached in the samples were 41 °C by the LIH and 43 °C by the TRIH. Both instruments successfully accomplished killing cancer cells, with minimal effects on normal cells. Conclusion: This work presents the first line of handheld medical induction heaters and have the potential to be a complement to existing cancer therapies. Significance: These instruments could enable the development of MFH modalities that will facilitate the clinical translation of this thermal treatment.