A genetic linkage map of nonheading Chinese cabbage
A genetic linkage map of nonheading Chinese cabbage
复制标题
不结球白菜遗传连锁图谱
DOI：
10.21273/jashs.132.6.816
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发表时间：
2007-11-01
影响因子：
1.9
通讯作者：
Hou, Xi-Lin
中科院分区：
文献类型：
--
作者：
Geng, Jian-Feng;Zhu, Cheng-Song;Hou, Xi-Lin
白菜(Brassica Rapa L.SSP.)中国草(L.)哈内尔特在中国中被称为不结球白菜，是东亚重要的蔬菜，其遗传改良需要遗传连锁图谱。以大白菜自交系Sw-3和SU-1 24为供试材料，利用育成的F-1杂交种蜀禄的112个加倍单倍体材料，构建了不结球白菜的首个遗传连锁图谱。138个分子标记被定位到14个连锁群。其中，序列相关扩增多态标记77个，简单序列重复标记27个，随机扩增多态DNA标记21个，简单序列重复标记13个。卡方检验表明，孟德尔分离比有54个标记发生扭曲，扭曲方向主要指向母本SW-3。这种扭曲不仅影响遗传距离的估计，而且还影响同一连锁群上扭曲标记的顺序。在给定特定的标记顺序的情况下，作者提出了一种多点方法来在F-2群体中以无偏的方式校正连锁图谱，同时考虑了扭曲标记、显性标记和缺失标记。使用一种新的方法来校正前面提到的加倍单倍体群体中的连锁图谱，该方法考虑了新的、扭曲的和缺失的标记。校正后的连锁图谱全长为1923.75 cM，平均标记间距为15.52 cM。该图谱将有助于选择育种和数量性状基因座的定位。
Brassica rapa L. ssp. chinensis (L.) Hanelt, known as nonheading chinese cabbage in China, is an important vegetable in eastern Asia and its genetic improvement requires a genetic linkage map. The first genetic linkage map of nonheading chinese cabbage using 112 doubled haploid lines derived from a released F-1 hybrid cultivar Shulu between two lines SW-3 and Su-1 24 was constructed in this paper. One hundred thirty-eight molecular markers were mapped into 14 linkage groups. Among these markers, there were 77 sequence-related amplified polymorphism markers, 27 simple sequence repeat markers, 21 random amplification polymorphic DNA markers, and 13 intersimple sequence repeat markers. Chi-square tests showed that 54 markers are distorted from Mendelian segregation ratios, and the direction of the distortion is mainly toward the maternal parent SW-3. The distortion affects not only the estimation of genetic distance, but also the order of distorted markers on a same linkage group. Given a specific marker order, the authors proposed a multipoint approach to correct the linkage map in an unbiased manner in an F-2 population while considering distorted, dominant, and missing markers. A new method was used to correct the linkage map in the doubled haploid population mentioned earlier considering new, distorted, and missing markers. The total length of the corrected linkage map was 1923.75 cM, with an average marker spacing of 15.52 cM. The map will facilitate selective breeding and mapping of quantitative trait loci.