MIGRATION OF FETAL INTESTINAL INTERVILLOUS CELLS IN NEONATAL MICE
MIGRATION OF FETAL INTESTINAL INTERVILLOUS CELLS IN NEONATAL MICE
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DOI：
10.1002/ar.1092270208
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发表时间：
1990-06-01
影响因子：
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通讯作者：
POTHIER, P
中科院分区：
文献类型：
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作者：
CALVERT, R;POTHIER, P
在新生小鼠中追踪在胎儿中标记的肠绒毛间上皮细胞的迁移。在妊娠17天时，第一组妊娠小鼠接受三次腹膜内注射3 H-胸苷（150 μ Ci/注射），每隔30分钟给药。在第一次注射后3小时处死两只母亲。在出生后第0、2、4、8、12、14和16天处死来自不同窝的小鼠。第二组妊娠小鼠在妊娠18 12天时注射，并在出生后第6、8、10、12、14和16天处死后代。将十二指肠和回肠段固定在戊二醛中，后固定在四氧化锇中，脱水，并包埋在Epon中。切片用醛品红染色并进行放射自显影。通过跟踪十二指肠和回肠最长绒毛中标记细胞的前缘和后缘，我们观察到：1）挤出区在出生后立即变得活跃，2）十二指肠中最长绒毛直到出生后10天和回肠中最长绒毛直到出生后14天才伸长，也就是说，当最初存在于胎儿中的所有标记上皮细胞都被挤出时。此外，通过测量妊娠17天的平滑肌内环层的内部界限与绒毛间上皮之间的距离（12.95 ± 0.001），发现妊娠17天的平滑肌内环层的内部界限与绒毛间上皮之间的距离（12.95 ± 0.001）。1.18在第3天（14.81 ± 0.51 μ m）或隐窝底部。0.91μ m），我们提出隐窝不发育为向下生长：而是绒毛间上皮被重塑并且隐窝-绒毛连接向上移动，远离外肌层。总之，通过分析新生小鼠中最长绒毛的生长模式，我们发现，在出生时在十二指肠和第4天在回肠中细胞产生和细胞挤出之间达到稳定状态。然而，在第10天之后，这种稳定状态将失去，从而使绒毛能够延长。
The migration of intestinal intervillous epithelial cells labeled in the fetus was followed in neonatal mice. At 17 days of gestation, a first group of pregnant mice received three intraperitoneal injections of 3H-thymidine (150 .mu.Ci/injection) administered at 30 min intervals. Two mothers were sacrificed 3 hours after the first injection. Mice from different litter were also sacrificed on days 0, 2, 4, 8, 12, 14, and 16 after birth. A second group of pregnant mice was injected at 18 1/2 days of gestation and offspring were sacrificed on days 6, 8, 10, 12, 14 and 16 after birth. Segments of duodenum and ileum were fixed in glutarldehyde, postfixed in osmium tetroxide, dehydrated, and embedded in Epon. Sections were stained with aldehyde fuchsin and processed for radioautography. By following the leading front and trailing edge of labeled cells in the longest villi of the duodenum and ileum, we observed that 1) extrusion zones become active immediately after birth and 2) the longest villi do not elongate until 10 days after birth in the duodenum and 14 days in the ileum, that is, when all labeled epithelial cells originally present in the fetus have been extruded. Moreover, by measuring the distance between the internal limit of the inner circular layer of smooth muscle and the intervillous epithelium at 17 days of gestation (12.95 .+-. 1.18 .mu.m) or the bottom of the crypts at day 3 (14.81 .+-. 0.91 .mu.m), we propose that crypts do not develop as downgrowths: rather the intervillous epithelium is reshaped and the crypt-villus junction moves upward, away from the muscularis externa. In conclusion, by analyzing the growth pattern of the longest villi in neonatal mice, we have found that a steady state between cell production and cell extrusion is reached at birth in the duodenum and by day 4 in the ileum. This steady state will be lost however, after day 10, thus enabling lengthening of the villi.