Identification of the sex-determining locus of Atlantic salmon (Salmo salar) on chromosome 2
Identification of the sex-determining locus of Atlantic salmon (Salmo salar) on chromosome 2
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DOI：
10.1159/000087528
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发表时间：
2006-01-01
影响因子：
1.7
通讯作者：
Davidson, WS
中科院分区：
文献类型：
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作者：
Artieri, CG;Mitchell, LA;Davidson, WS
为了更好地了解大西洋鲑性别决定基因SEX，我们综合了连锁作图、物理作图和染色体核型分析的数据。SEX已被映射到大西洋鲑鱼连锁群1（ASL 1），并与几个微卫星标记。我们已经使用从这些性连锁微卫星标记的侧翼区域设计的探针来筛选细菌人工染色体（BAC）文库，其代表大西洋鲑鱼基因组的11.7 X覆盖率，其已经被HindIII指纹识别并组装成重叠群。已经鉴定了含有性连锁微卫星及其相关重叠群的BAC，并且通过荧光原位杂交（FISH）将代表性BAC置于大西洋鲑鱼染色体上。这确定了2号染色体，一个大的中着丝粒，作为性染色体。通过FISH在该染色体上定位几个BAC，可以相对于染色体2定位ASL 1。含有SEX的区域似乎位于标记Ssa202DU和通过DAPI染色鉴定的异染色质区域之间的长臂上。性连锁重叠群内克隆的BAC末端测序揭示了性染色体上五个迄今为止未定位的基因沿着.我们正在使用计算机模拟方法结合BAC文库的物理探测来扩展BAC重叠群以提供ASL 1的物理图谱，以期对2号染色体进行测序，并在此过程中鉴定性别决定基因。版权所有（c）2006 S. Karger AG，巴塞尔
We have integrated data from linkage mapping, physical mapping and karyotyping to gain a better understanding of the sex- determining locus, SEX, in Atlantic salmon (Salmo salar). SEX has been mapped to Atlantic salmon linkage group 1 (ASL1) and is associated with several microsatellite markers. We have used probes designed from the flanking regions of these sex- linked microsatellite markers to screen a bacterial artificial chromosome (BAC) library, representing an 11.7 x coverage of the Atlantic salmon genome, which has been HindIII fingerprinted and assembled into contigs. BACs containing sex- linked microsatellites and their related contigs have been identified and representative BACs have been placed on the Atlantic salmon chromosomes by fluorescent in situ hybridization (FISH). This identified chromosome 2, a large metacentric, as the sex chromosome. By positioning several BACs on this chromosome by FISH, it was possible to orient ASL1 with respect to chromosome 2. The region containing SEX appears to lie on the long arm between marker Ssa202DU and a region of heterochromatin identified by DAPI staining. BAC end- sequencing of clones within sex- linked contigs revealed five hitherto unmapped genes along the sex chromosome. We are using an in silico approach coupled with physical probing of the BAC library to extend the BAC contigs to provide a physical map of ASL1, with a view to sequencing chromosome 2 and, in the process, identifying the sex- determining gene. Copyright (c) 2006 S. Karger AG, Basel