The effects of blue and red light color combinations on the growth and immune performance of juvenile steelhead trout, Oncorhynchus mykiss
The effects of blue and red light color combinations on the growth and immune performance of juvenile steelhead trout, Oncorhynchus mykiss
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蓝光和红光颜色组合对硬头鳟幼鱼生长和免疫性能的影响
DOI：
10.1016/j.aqrep.2022.101156
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发表时间：
2022-05-14
影响因子：
3.7
通讯作者：
Dong, Shuanglin
中科院分区：
文献类型：
--
作者：
Chen, Xueweijie;Zhou, Yangen;Dong, Shuanglin
为了研究虹鳟鱼（Oncorhynchus mykiss）在不同光色条件下的生长和免疫应答，我们研究了不同红、蓝光组合对虹鳟幼鱼（初始体重：34.67± 2.69 g）血浆非特异性免疫、肝脏抗氧化能力和GH/IGF-1轴基因表达的影响。为期16周的试验设置了6个处理，其中光色在150 lx的恒定光强下变化：12 h白色光+12 h黑暗（12 W），12 h蓝光+12 h黑暗（12 B），12 h红光+12 h黑暗（12 R），1.5 h蓝光+9 h红光+1.5 h蓝光+12 h黑暗（3B 9 R），3 h蓝光+6 h红光+3 h蓝光+12 h黑暗（6 B6 R），共12 h蓝红光+12 h黑暗（T12 BR）。结果表明，12 B和3B 9 R光环境显着提高血浆免疫球蛋白M水平在虹鳟鱼，和3B 9 R光环境也促进肝生长激素受体，胰岛素样生长因子1，胰岛素样生长因子结合蛋白的基因表达显着增加。3B 9 R光环境也被发现通过增加肝脏超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性来增强鳟鱼的抗氧化能力。相比之下，鳟鱼暴露于6 B6 R光环境显示出显着的增长和免疫性能的下降，并有类似的肝脏抗氧化能力的下降。此外，发现暴露于12 R光环境的鱼的特征在于脂质过氧化和丙二醛水平升高，这是肝应激的指示。此外，我们观察到，在响应光的变化，有升高的活动中的谷氨酸草酰乙酸转氨酶和乳酸脱氢酶的血浆中的虹鳟鱼，从而表明心肌细胞功能的抑制。综上所述，我们的研究结果表明，3B 9 R光环境模拟了自然水下环境中蓝-红-蓝的光色变化的日节律，这有利于提高虹鳟的生长和免疫力，因此可以用于为虹鳟生产提供合适的光照条件。但是，在鳟鱼养殖中应避免光色的突然变化。
To examine the growth and immune response of steelhead trout (Oncorhynchus mykiss) under conditions of differing light color, we investigated the effects of different red and blue light color combinations on plasma non-specific immunity, hepatic antioxidant capacity, and GH/IGF-1 axis gene expression in juvenile steelhead trout (initial weight: 34.67 & PLUSMN; 2.69 g). The 16-week trial was set up with six treatments in which light color varied at a constant light intensity of 150 lx: 12 h white light + 12 h dark (12 W), 12 h blue light + 12 h dark (12B), 12 h red light + 12 h dark (12 R), 1.5 h blue light + 9 h red light + 1.5 h blue light + 12 h dark (3B9R), 3 h blue light + 6 h red light + 3 h blue light + 12 h dark (6B6R), total 12 h of blue and red light + 12 h dark (T12BR). The results revealed that the 12B and 3B9R light environments significantly enhanced plasma immunoglobulin M levels in steelhead trout, and that the 3B9R light environment also promoted significant increases in the gene expression of hepatic growth hormone receptor, insulin-like growth factor 1, and insulin-like growth factor-binding protein. The 3B9R light environment was also found enhance the antioxidant capacity of trout by increasing hepatic superoxide dismutase, catalase, and glutathione peroxidase activities. In contrast, trout exposed to the 6B6R light environment showed significant reductions in growth and immune performance, and there were similar reductions in hepatic antioxidant capacity. Furthermore, fish exposed to the 12R light environment were found to be characterized by elevated levels of lipid peroxidation and malondialdehyde, which are indicative of hepatic stress. In addition, we observed that in response to light variation, there were elevations in the activities of glutamic oxaloacetic transaminase and lactic dehydrogenase in the plasma of steelhead trout, thereby indicating an inhibition of cardiomyocyte function. In summary, our findings indicate that a 3B9R light environment simulates the blue-red-blue daily rhythm of light color variation in natural underwater environments, which is beneficial with respect to enhancing the growth and immunity of steelhead trout, and could accordingly be used to provide suitable light conditions in commercial trout production. However, sudden changes in light color should be avoided in the aquaculture of trout.