Stoichiometry of BkdR to substrate DNA in Pseudomonas putida.
Stoichiometry of BkdR to substrate DNA in Pseudomonas putida.
复制标题
恶臭假单胞菌中 BkdR 与底物 DNA 的化学计量。
DOI：
10.1006/bbrc.1996.0891
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Sokatch,JR
中科院分区：
文献类型：
--
作者：
Huang,N;Madhusudhan,KT;Sokatch,JR
BkdR is the transcriptional activator of thebkdoperon ofPseudomonas putida,which encodes branched chain keto acid dehydrogenase. BkdR binds to a large region of DNA between its own structural gene and the first gene of thebkdoperon. The object of the present studies was to determine the stoichiometry of binding as part of an effort to understand the action of BkdR in regulation of thebkdoperon. [35S]BkdR was prepared and found to be essentially 100% active in the gel shift assay. Only one complex was formed under all the conditions used. The stoichiometry of BkdR binding to its specific substrate DNA was three tetramers per mole substrate DNA. L-valine did not affect the stoichiometry although this ligand was previously shown to affect the DNase I protection pattern. The addition of nonspecific DNA to the incubation mixture also did not affect this stoichiometry.
