Hydroxyproline titers in gingival crevicular fluid.
Hydroxyproline titers in gingival crevicular fluid.
复制标题
龈沟液中的羟脯氨酸滴度。
DOI：
10.1111/j.1600-0765.1987.tb01570.x
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发表时间：
1987
影响因子：
3.5
通讯作者：
Svanberg,GK
中科院分区：
文献类型：
--
作者：
Svanberg,GK
炎症和细胞外基质降解的增加导致胶原蛋白的净损失是牙周病发病和进展的特征。白细胞的积累和水解酶的产生增加导致牙周病变前沿的胶原蛋白损失60 - 70%（1）。大部分分解产物被牙龈脉管系统的炎性渗出物从组织中冲洗出来（2），出现在龈沟液（GCF）中。因此，定量的GCF中的分解产物应该是一个有用的工具，以监测牙龈结缔组织中的细胞外基质的损失。胶原蛋白的降解可以通过释放羟脯氨酸（Hyp）来监测，羟脯氨酸（Hyp）占该蛋白质的约10%。Clq是第一补体因子的亚组分，是Hyp的唯一其他重要来源。它含有4.3%的羟脯氨酸（重量）（3），并在慢性牙周炎患者的牙龈缝隙中发现涂层细菌（4）。因此，GCF中胶原衍生Hyp的测定需要消除Clq衍生Hyp。已经建立了1至2/< 1体积GCF中胶原衍生Hyp的测定（5），并用于分析比格犬实验性牙周病发作和进展期间的样品。在3只牙周组织健康的雌性比格犬中，通过避免所有口腔卫生措施来引发实验性牙龈炎。每周一次，用微毛细管从10个下颌前磨牙收集约1.5 μ l体积的GCF。8 wk后，用棉花线绕牙颈部，放置9d。GCF取样4天后，去除结扎，随后在每周的时间间隔。每次采集血液并进行处理，
Infiammation and increased degradation of extracellular matrix resulting in a net loss of collagen are characteristic features of the onset and progression of periodontal diseases. The accumulation of leukocytes and an increased production of hydrolytic enzymes results in a 60 to 70% loss of collagen at the advancing front of the periodontal lesion (1).Most of the breakdown products are washed out of the tissue by the infiammatory exudate from the gingival vasculature (2) to appear in the gingival crevicular fiuid (GCF). Therefore, the quantification of the breakdown products in the GCF should be a useful tool to monitor loss of extracellular matrix in the gingival connective tissue. Degradation of collagen can be monitored by the release of hydroxyprohne (Hyp) which accounts for about 10% of this protein. Clq, a subcomponent of the first complement factor, is the only other significant source of Hyp. It contains 4.3% Hyp by weight (3) and is found coating bacteria in the gingival crevices of patients with chronic periodontitis (4). Consequently, an assay for collagen-derived Hyp in GCF requires the elimination of Clq-derived Hyp. An assay for collagen-derived Hyp in 1 to 2/< 1 volumes of GCF has been established (5) and was used for analysis of samples during the onset and progression of experimental periodontal disease in the beagle dog. Experimental gingivitis was initiated in 3 female beagle dogs with a healthy periodontium by refraining from all oral hygiene measures. Approximately 1.5//I volumes of GCF were collected from 10 mandibular premoiars with microcapillary tubes once every week. After 8 wk, cotton floss was applied around the neck of the teeth and left in place for 9 d. GCF was sampled 4 d after the removal of the ligatures and subsequently at weekly intervals. Blood was collected on each occasion and processed for