CORRECTED RETENTION INDEXES IN HPLC - THEIR USE FOR THE IDENTIFICATION OF ACIDIC AND NEUTRAL-DRUGS
CORRECTED RETENTION INDEXES IN HPLC - THEIR USE FOR THE IDENTIFICATION OF ACIDIC AND NEUTRAL-DRUGS
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DOI：
10.1093/jat/12.2.67
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发表时间：
1988-03-01
影响因子：
2.5
通讯作者：
ADERJAN, R
中科院分区：
文献类型：
--
作者：
BOGUSZ, M;ADERJAN, R
采用两步梯度洗脱和9种不同OD硅胶色谱柱，对37种选定的酸性和中性药物进行了分析。根据一系列烷基芳基酮计算药物的保留指数值(RI)，并根据前面描述的程序进行校正。校正过程降低了RI值的柱间变异性，校正后参考柱和其他柱获得的RI值之间的差异显著减小。对9个柱上分析的所有药物计算的未校正RI值的平均标准偏差为25个RI单位(范围为8至40)。校正后，平均标准差为15 RI单位(范围3~26)。该方法适用于商品不同的ODS型硅胶色谱柱的结果比较。
Thirty-seven selected acidic and neutral drugs were analyzed by HPLC with two-step gradient elution and nine different OD silica columns. The retention index values (RI) for drugs were calculated against a series of alkyl-arylketones and corrected according to the procedure described earlier. The correction procedure decreased the intercolumn variability of the RI values, and the differences between the RI values obtained for the reference column and other columns substantially declined after correction. The mean standard deviation of uncorrected RI values, calculated for all drugs analyzed on 9 columns, amounted to 25 RI units (range 8 to 40). After correction, the mean standard deviation was 15 RI units (range 3 to 26). The method proved to be suitable for the comparison of results obtained with commercially different ODS silica columns.