Pulsed diode laser-based singlet oxygen monitor for photodynamic therapy: in vivo studies of tumor-laden rats
Pulsed diode laser-based singlet oxygen monitor for photodynamic therapy: in vivo studies of tumor-laden rats
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DOI：
10.1117/1.3042265
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发表时间：
2008-11-01
影响因子：
3.5
通讯作者：
Hasan, Tayyaba
中科院分区：
文献类型：
--
作者：
Lee, Seonkyung;Vu, Danthu H.;Hasan, Tayyaba
光动力疗法（PDT）是一种有前途的癌症治疗，其涉及光敏剂的光激发，所述光敏剂促进氧分子至亚稳态O-2（a（1）Delta）状态（单线态氧）。据信该物种负责PDT期间癌细胞的破坏。我们描述了一个光纤耦合，脉冲二极管激光器为基础的诊断单重态氧。我们使用时间和光谱滤波，以提高检测的弱O-2（a -> X）发射近1.27 μ m。我们目前的数据表明，实时单线态氧的生产在荷瘤大鼠与二氢卟啉e6和5-氨基乙酰丙酸诱导的原卟啉光敏剂。我们还观察到PDT治疗后肿瘤消退与测量的单线态氧的相对量之间的正相关性。这些结果是有希望的传感器作为PDT的实时剂量计的发展。(c)2008年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。[DOI 10.1117/1.3042265]
Photodynamic therapy (PDT) is a promising cancer treatment that involves optical excitation of photosensitizers that promote oxygen molecules to the metastable O-2(a(1)Delta) state (singlet oxygen). This species is believed to be responsible for the destruction of cancerous cells during PDT. We describe a fiber optic-coupled, pulsed diode laser-based diagnostic for singlet oxygen. We use both temporal and spectral filtering to enhance the detection of the weak O-2(a -> X) emission near 1.27 mu m. We present data that demonstrate real-time singlet oxygen production in tumor-laden rats with chlorin e6 and 5-aminolevulinic acid-induced protoporphyrin photosensitizers. We also observe a positive correlation between post-PDT treatment regression of the tumors and the relative amount of singlet oxygen measured. These results are promising for the development of the sensor as a real-time dosimeter for PDT. (c) 2008 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3042265]