Immunoglobutin deposits in liver tissue from infants with biliary atresia and the correlation to cytomegatovirus infection
Immunoglobutin deposits in liver tissue from infants with biliary atresia and the correlation to cytomegatovirus infection
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DOI：
10.1016/j.jpedsurg.2004.11.035
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发表时间：
2005-03-01
影响因子：
2.4
通讯作者：
Papadogiannakis, N
中科院分区：
文献类型：
--
作者：
Fischler, B;Woxenius, S;Papadogiannakis, N
目的：本报告的目的是研究免疫球蛋白沉积的数量和分布在肝活检从婴儿胆道闭锁（BA）和相关的结果与巨细胞病毒（CMV）感染的status.Methods：储存肝活检18例BA和6例对照组无肝病患者免疫化学染色检测IgG和IgM沉积。免疫球蛋白染色的强度通过半定量评分量表进行评价。正在进行的CMV感染被定义为CMV-IgM检测血清和/或CMV的分离在尿液中，并注意到在9例BA。结果：当分析Inumoglobulin沉积在肝细胞小管膜上的IgM沉积的强度评分显着较高的活检组织中，从BA感染CMV的患者比那些没有。对照活检组织中未检测到小管染色。结论：结果支持免疫机制在BA发病机制中具有重要意义的可能性，CMV感染可能触发这种机制。(c)2005年爱思唯尔公司All rights reserved.
Purpose: The aim of this report was to study the amount and distribution of immunoglobulin deposits in liver biopsies from infants with biliary atresia (BA) and correlate the results to the cytomegalovirus (CMV) infection status.Methods: Stored liver biopsies from 18 patients with BA and from 6 control patients without liver disease were immunohistochemically stained to detect IgG and IgM deposits. The intensity of the immunoglobulin staining was evaluated by a semiquantitative scoring scale. Ongoing CMV infection was defined as the detection of CMV-IgM in serum and/or the isolation of CMV in the urine and was noted in 9 of the patients with BA.Results: When analyzing the inummoglobulin deposits on the hepatocellular canalicular membrane the intensity score for IgM deposits was significantly higher in biopsies from patients with BA infected with CMV than in those without. No canalicular staining was detected in control biopsies.Conclusions: The results support the possibility that immunologic mechanisms are of importance in the pathogenesis of BA and that a CMV infection may trigger such mechanisms. (c) 2005 Elsevier Inc. All rights reserved.