Vacuum UV laser induced scission of simian virus 40 DNA.
Vacuum UV laser induced scission of simian virus 40 DNA.
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真空紫外激光诱导猿猴病毒 40 DNA 断裂。
DOI：
10.1111/j.1751-1097.1984.tb03398.x
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发表时间：
1984
影响因子：
3.3
通讯作者：
George,J
中科院分区：
文献类型：
--
作者：
Johnson-Thompson,M;Halpern,JB;Jackson,WM;George,J
本文研究了真空紫外光照射对猿猴病毒40(SV40)DNA I的影响。用ArF激光(LD=193 nm)照射SV40DNA I后，双链超螺旋分子(DNA I)的任何一条链都发生单链断裂，并转化为DNA II构象。随着照射时间的增加，已经断裂的链会发生额外的断裂，通过碱性蔗糖离心法分析，会产生更小的碎片。这些结果表明，短时间的照射会导致SV40DNA I的构象改变。
The effect of vacuum UV‐laser irradiation on Simian virus 40 (SV40) DNA I was studiedin vitro. Following exposure of SV40 DNA I to an argon fluoride (ArF) laser (LD = 193 nm), single‐strand breaks occur in either strand of the double‐stranded superhelical molecule (DNA I) and convert it to the DNA II conformation. Upon increased irradiation times, additional breaks occur in already scissioned strands resulting in the production of smaller fragments as analyzed by alkaline sucrose centrifugation. These results suggest that short periods of irradiation lead to conformational alterations of SV40 DNA I.