Degradation of 1,1,1-trichloro-2,2-bis (4-chlorophenyl) ethane (DDT) by brown-rot fungi
Degradation of 1,1,1-trichloro-2,2-bis (4-chlorophenyl) ethane (DDT) by brown-rot fungi
复制标题
DOI：
10.1263/jbb.105.614
复制
发表时间：
2008-06-01
影响因子：
2.8
通讯作者：
Kondo, Ryuichiro
中科院分区：
文献类型：
--
作者：
Purnomo, Adi Setyo;Kamei, Ichiro;Kondo, Ryuichiro
研究了12种褐腐真菌（BRF）对1，1，1-三氯-2，2-二（4-氯苯基）乙烷（DDT）的降解能力。在马铃薯葡萄糖肉汤（PDB）培养基中，密粘褶菌、松拟层孔菌和狄氏代达莱表现出较强的降解滴滴涕的能力。在PDB培养基中，G trabeum降解DDT的代谢产物为1，1-二氯-2，2-双（4-氯苯基）乙烷（DDD）、1，1-二氯-2，2-双（4-氯苯基）乙烯（DDE）和4，4-二氯二苯甲酮（DBP）。DDT在密革兰氏菌中的降解途径不同于细菌和其他真菌中的降解途径，特别是在DDE向DDD的转化方面。另一方面，DBP没有被检测到作为DDT降解的代谢产物在FeSO 4-缺乏培养的G trabeum，而DDE和DDD被检测到，表明参与了铁依赖性反应。只有DBP检测到DDT，DDE和DDD降解通过化学芬顿反应的条件下类似的G trabeum文化。这些化合物可以通过芬顿反应直接转化为DBP。
Twelve species of brown-rot fungi (BRF) have been investigated for their ability to degrade 1,1,1-trichloro-2,2-bis (4-chlorophenyl) ethane (DDT). In potato dextrose broth (PDB) medium, Gloeophyllum trabeum, Fomitopsis pinicola and Daedalea dickinsii showed a high ability to degrade DDT. 1,1- Dichloro-2,2-bis (4-chlorophenyl) ethane (DDD), 1,1-dichloro-2,2-bis (4-chlorophenyl)ethylene (DDE) and 4,4-dichlorobenzophenone (DBP) were detected as metabolic products of DDT degradation by G trabeum in PDB medium. The DDT degradation pathway in G trabeum is proposed, which differs from the proposed pathways in bacteria and other fungi, particularly in the transformation of DDE to DDD. On the other hand, DBP was not detected as a metabolic product of DDT degradation in FeSO4-deficient cultures of G trabeum, whereas DDE and DDD were detected, indicating the involvement of an iron-dependent reaction. Only DBP was detected from DDT, DDE and DDD degradation via a chemical Fenton reaction under conditions similar to those in G trabeum cultures. These compounds may be directly transformed to DBP via a Fenton reaction.