Functional expression of aquaporins in embryonic, postnatal, and adult mouse lenses
Functional expression of aquaporins in embryonic, postnatal, and adult mouse lenses
复制标题
DOI：
10.1002/dvdy.21125
复制
发表时间：
2007-05-01
影响因子：
2.5
通讯作者：
Mathias, Richard T.
中科院分区：
文献类型：
--
作者：
Varadaraj, Kulandaiappan;Kumari, Sindhu S.;Mathias, Richard T.
水通道蛋白0(AQP0)和AQP1在晶状体中表达，每种细胞类型不同，其功能作用尚不完全清楚。我们先前的研究表明，这两种水通道蛋白具有水转运蛋白的功能。为了进一步了解这两种不同的水通道蛋白在晶状体中的功能意义，我们研究了它们的启动和持续表达。AQP0转录本和蛋白在胚胎期(E)11.25在发育中的晶状体初级纤维细胞中首次检测到，AQP0的合成在次生纤维细胞中持续到成年期。AQP1在胚胎17.5天开始在晶状体前上皮细胞中低水平表达，在出生后6.5天逐渐向赤道上皮细胞表达。出生后晶状体上皮细胞膜透水性和晶状体透明度的增加与AQP1表达的增加相一致。AQP1的表达在P30达到高峰，并持续到成人期，在前上皮和赤道上皮细胞中都是如此。AQP1表达的增强伴随着晶状体大小的增加，提示晶状体微循环系统的建立进展。体外和体内研究表明，这两种水通道蛋白都至少有一个重要的功能，那就是晶状体微循环系统中的水运输。然而，这两个水通道蛋白的时间表达表明了在晶状体发育和透明度方面的明显独特作用/S。据我们所知，这是首次报道AQP0和AQP1在晶状体发育和分化过程中的表达模式及其与晶状体透明度的关系。《发展动力学》236；1319-1328,2007。(C)2007年Wiley-Liss，Inc.
Aquaporin 0 (AQP0) and AQP1 are expressed in the lens, each in a different cell type, and their functional roles are not thoroughly understood. Our previous study showed that these two AQPs function as water transporters. In order to further understand the functional significance of these two different aquaporins in the lens, we investigated their initiation and continued expression. AQP0 transcript and protein were first detected at embryonic stage (E) 11.25 in the differentiating primary fiber cells of the developing lens; its synthesis continued through the adult stage in the secondary fiber cells. Low levels of AQP1 expression were first seen in lens anterior epithelial cells at E17.5; following postnatal day (P) 6.5, the expression gradually progressed towards the equatorial epithelial cells. In the postnatal lens, the increase in membrane water permeability of epithelial cells and lens transparency coincides with the increase in AQP1 expression. AQP1 expression reaches its peak at P30 and continues through the adult stage both in the anterior and equatorial epithelial cells. The enhancement in AQP1 expression concomitant with the increase in the size of the lens suggests the progression in the establishment of the lens microcirculatory system. In vitro and in vivo studies show that both aquaporins share at least one important function, which is water transport in the lens microcirculatory system. However, the temporal expression of these two AQPs suggests an apparently unique role/s in lens development and transparency. To our knowledge, this is the first report on the expression patterns of AQP0 and AQP1 during lens development and differentiation and their relation to lens transparency. Developmental Dynamics 236;1319-1328, 2007. (C) 2007 Wiley-Liss, Inc.