Evidence of enhanced non-enzymatic generation of nitric oxide on the skin surface of acupuncture points: An innovative approach in humans
Evidence of enhanced non-enzymatic generation of nitric oxide on the skin surface of acupuncture points: An innovative approach in humans
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DOI：
10.1016/j.niox.2007.05.004
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发表时间：
2007-09-01
影响因子：
3.9
通讯作者：
Pandjaitan, Marintan
中科院分区：
文献类型：
--
作者：
Ma, Sheng-Xing;Li, Xi-Yan;Pandjaitan, Marintan
本研究对穴位(穴位)皮肤表面采集的总硝酸盐和亚硝酸盐(NOx-)进行了量化，并确定了穴位上一氧化氮(NO)的化学生成是否与细菌非酶还原硝酸盐有关。在50名健康志愿者的前臂或腿上缠上一根纵向切成两半的小塑料管(0.5×7厘米)。将含有NO清除化合物、血红蛋白或2-phenyl-4，4，5，5-tetramethylimidazoline-1-oxyl 3-氧化物的NO收集液放置在附着在皮肤表面的管子内20分钟。用化学发光法对采集的样品中NOx-的浓度进行了定量。与非经络控制区相比，心包经络4个穴位和膀胱经2个穴位的NOx浓度显著升高。在穴位采集的前20min，NOx水平的时间间隔显著高于对照组，但在20-40、40-60和60-80min采集的研究组之间的浓度相似。与水处理相比，次氯酸钠对皮肤表面的NOx-浓度和细菌菌落数有明显的降低作用。这是第一个通过非侵入性设备在人体皮肤穴位上成功量化一氧化氮的证据。我们的结论是，皮肤表面的NO是从皮肤表面生理性地释放出来的，在穴位处水平较高，除了L精氨酸来源的NO合成外，细菌对硝酸盐的非酶还原也参与了皮肤穴位上NO的化学生成。(C)2007 Elsevier Inc.保留所有权利。
The present study quantified total nitrate and nitrite (NOx-,) collected from the skin surface along acupuncture points (acupoints) and determined whether non-enzymatic reduction of nitrate by bacteria is involved in chemical generation of nitric oxide (NO) on acupoints. A small plastic tube (0.5 x 7 cm) cut in half lengthwise was taped to the forearm or leg in 50 healthy volunteers. NO-collecting solutions with NO-scavenging compounds, hemoglobin or 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide, was placed inside the tubing attached to the skin surface for 20 min. The concentrations of NOx- in the collected samples were quantified by using chemiluminescence. NOx- concentration was significantly enhanced in four acupoints on the pericardium meridian and in two acupoints on the bladder meridian compared with those collected on non-meridian control areas. The time intervals of NOx- levels were significantly higher at the first 20 min of acupoint collection, but the concentrations were similar among the study groups collected at 20-40, 40-60, and 60-80 min. NOx- concentrations and numbers of bacteria colonies detected on the skin surface were markedly reduced by pretreatment of skin with sodium hypochlorite compared to water treatment. This is the first evidence showing that NO has been successfully quantified on skin acupoints by a non-invasive device in humans. We conclude that NO is physiologically released from the skin surface with a higher level at acupoints, and that the non-enzymatic reduction of nitrate by bacteria is involved in chemical generation of NO on skin acupoints in addition to L-arginine-derived NO synthesis. (c) 2007 Elsevier Inc. All rights reserved.