Contrast-agent gas bodies enhance hemolysis induced by lithotripter shock waves and high-intensity focused ultrasound in whole blood
Contrast-agent gas bodies enhance hemolysis induced by lithotripter shock waves and high-intensity focused ultrasound in whole blood
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DOI：
10.1016/s0301-5629(96)00126-3
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发表时间：
1996-01-01
影响因子：
2.9
通讯作者：
Thomas, RM
中科院分区：
文献类型：
--
作者：
Miller, DL;Thomas, RM
将新鲜犬全血置于1.3 mL一次性移液管球中，加入或不加入Albunex造影剂，暴露于碎石机冲击波（频率：2 Hz;平均压力振幅：14.8 MPa）或1.28 MHz高强度聚焦超声，平均压力振幅高达17.8 MPa，在500次或更多次冲击波后，未添加造影剂，200次冲击波后添加1%造影剂，100次冲击波后添加10%造影剂，可检测到高达约10%的显著冲击波诱导溶血，加入造影剂后溶血有所增加，但这种增强无统计学意义。对于超声暴露，在17.8 MPa下连续暴露100 ms，未加入气体体时，检测到接近100%的明显溶血。加入1%造影剂通过将表观阈值降低至10 MPa而增强溶血，具有100 ms总开启时间（20 μ s、100 μ s和1 ms突发）的突发模式暴露也产生了添加造影剂的溶血增强，结果与100 ms连续暴露相似。添加的气体体似乎通过充当血液中的空化核来降低空化阈值。版权所有（C）1996世界医学和生物学超声联合会。
Fresh canine whole blood was exposed in 1.3-mL disposable pipette bulbs with or without added Albunex contrast agent to lithotripter shock waves (rate: 2 Hz; mean pressure amplitude: 14.8 MPa) or 1.28-MHz high-intensity focused ultrasound up to a mean pressure amplitude of 17.8 MPa, Significant shock-wave-induced hemolysis up to about 10% was detected after 500 or more shock waves without added agent, after 200 with 1% added agent and after 100 with 10% added contrast agent, Hemolysis was increased somewhat with added contrast agent, but this enhancement was not statistically significant, For ultrasound exposure, significant hemolysis approaching 100% was detected for 100-ms continuous exposure at 17.8 MPa without added gas bodies, Addition of 1% contrast agent enhanced the hemolysis by reducing the apparent threshold to 10 MPa, Burst mode exposure with a 100-ms total on-time (20 mu s, 100 mu s and 1-ms bursts) also yielded hemolysis enhancement with added contrast agent, with results similar to the 100-ms continuous exposure, The added gas bodies appear to lower cavitation thresholds by serving as cavitation nuclei in the blood. Copyright (C) 1996 World Federation for Ultrasound in Medicine & Biology.