In vivo import of a normal or mutagenized heterologous transfer RNA into the mitochondria of transgenic plants: towards novel ways of influencing mitochondrial gene expression?
In vivo import of a normal or mutagenized heterologous transfer RNA into the mitochondria of transgenic plants: towards novel ways of influencing mitochondrial gene expression?
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将正常或诱变的异源转移RNA体内导入转基因植物的线粒体：寻找影响线粒体基因表达的新方法？
DOI：
10.1002/j.1460-2075.1992.tb05172.x
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
A. Dietrich
中科院分区：
文献类型：
--
作者：
I. Small;L. Marechal;J. Masson;G. Pelletier;A. Cosset;J. Weil;A. Dietrich
多种生物体都报道了核编码 RNA 存在于线粒体内的证据，并且推测这是由于特定胞质 RNA 的输入所致。在植物中，第一个例子是豆类的线粒体亮氨酸转移 RNA。在所有情况下，证据都是间接的，基于线粒体 RNA 与核而非线粒体 DNA 的杂交。在这里，我们表明，携带来自豆核DNA的亮氨酸tRNA基因的转基因马铃薯植物在细胞质和线粒体内部都含有从引入的基因转录的RNA，这提供了线粒体亮氨酸tRNA源自核基因并导入线粒体的证据。在反密码子环中插入 4 bp 的相同豆类基因也在转基因马铃薯植物中表达，并且发现转录本存在于线粒体内，表明这种天然 RNA 导入系统最终可用于将外源 RNA 序列引入线粒体。
Evidence that nuclear‐encoded RNAs are present inside mitochondria has been reported from a wide variety of organisms, and is presumed to be due to import of specific cytosolic RNAs. In plants, the first examples were the mitochondrial leucine transfer RNAs of bean. In all cases, the evidence is circumstantial, based on hybridization of the mitochondrial RNAs to nuclear and not mitochondrial DNA. Here we show that transgenic potato plants carrying a leucine tRNA gene from bean nuclear DNA contain RNA transcribed from the introduced gene both in the cytosol and inside mitochondria, providing proof that the mitochondrial leucine tRNA is derived from a nuclear gene and imported into the mitochondria. The same bean gene carrying a 4 bp insertion in the anticodon loop was also expressed in transgenic potato plants and the transcript found to be present inside mitochondria, suggesting that this natural RNA import system could eventually be used to introduce foreign RNA sequences into mitochondria.