Simultaneous determination of the levels of deoxynivalenol, 3-acetyldeoxynivalenol, and nivalenol in grain and feed samples from South Korea using a high-performance liquid chromatography-photodiode array detector
Simultaneous determination of the levels of deoxynivalenol, 3-acetyldeoxynivalenol, and nivalenol in grain and feed samples from South Korea using a high-performance liquid chromatography-photodiode array detector
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DOI：
10.1007/s13765-016-0238-8
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发表时间：
2016-12-01
影响因子：
3.2
通讯作者：
Chung, Soo Hyun
中科院分区：
文献类型：
--
作者：
Kim, Dong-Ho;Hong, Sung-Yong;Chung, Soo Hyun
建立了同时分析谷物和饲料中脱氧雪腐镰刀菌醇（DON）、雪腐镰刀菌醇（NIV）和3-乙酰脱氧雪腐镰刀菌醇（3AcDON）的高效液相色谱法。方法对DON、3AcDON和NIV的定量限分别为11.0、27.6和13.5 ng/g。对韩国采集的300份谷物和饲料样品进行HPLC分析发现，DON和NIV在玉米中的发病率分别为22.5%和7.5%，在水稻中的发病率分别为4.0和54.0%。混合颗粒粉中DON和NIV的发生率分别为62.0和24.0%。我们的研究还表明，动物饲料中主要污染的DON（98.1%）在32.8 ~ 950.25 ng/g范围内，平均浓度为353.32 ng/g。粮食中DON的含量没有超过食品医药品安全厅规定的最高限量（每公斤1毫克）。
An HPLC method combined with immunoaffinity clean-up was developed for the simultaneous analysis of deoxynivalenol (DON), nivalenol (NIV), and 3-acetyldeoxynivalenol (3AcDON) in grains and feeds. The limits of quantification of the method for DON, 3AcDON, and NIV were 11.0, 27.6, and 13.5 ng/g, respectively. HPLC analyses of 300 samples of grains and feeds collected in South Korea showed that the incidence of DON and NIV in corn was 22.5 and 7.5%, while that in rice was 4.0 and 54.0%, respectively. The incidence of DON and NIV in mixed grain powder was 62.0 and 24.0%, respectively. Our study also showed that animal feeds were mainly contaminated with DON (98.1%) in the range of 32.8-950.25 ng/g with the mean concentration of 353.32 ng/g. The levels of DON in grains did not exceed the maximum allowable limit (1 mg/kg) set by the Korean Food and Drug Administration.