A Web-Based Platform for Designing Vaccines against Existing and Emerging Strains of Mycobacterium tuberculosis.
A Web-Based Platform for Designing Vaccines against Existing and Emerging Strains of Mycobacterium tuberculosis.
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DOI：
10.1371/journal.pone.0153771
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Raghava GP
中科院分区：
文献类型：
--
作者：
Dhanda SK;Vir P;Singla D;Gupta S;Kumar S;Raghava GP
开发抗耐药结核分枝杆菌（Mtb）的有效疫苗对于挽救每年因结核病导致的数百万人过早死亡至关重要。这篇论文描述了一个为帮助研究人员使用传统和现代方法设计针对新出现的结核分枝杆菌菌株的疫苗而开发的门户网站。首先，我们对59个分枝杆菌物种的基因组进行了注释，以了解结核样、非结核样和疫苗株在基因组水平上的相似性/差异性。其次，在每个结核分枝杆菌菌株中预测了基于抗原的候选疫苗。第三，在上述抗原候选疫苗中预测/发现了基于表位的候选疫苗，可以刺激免疫系统的所有手臂。最后，在抗原水平上为上述菌株开发了一个预测候选疫苗的表位数据库。为了设计针对新测序的Mtb菌株基因组的疫苗，server集成了三个模块，用于鉴定菌株，抗原，表位特异性候选疫苗。我们观察到103522个独特的肽（9mers）具有诱导抗体反应和/或混杂结合MHC等位基因和/或具有刺激T淋巴细胞的能力的潜力。总之，这个门户网站将对致力于设计抗结核分枝杆菌疫苗（包括耐药菌株）的研究人员有用。可用性：该数据库可在http://crdd.osdd.net/raghava/mtbveb/免费获得。
Development of an effective vaccine against drug-resistant Mycobacterium tuberculosis (Mtb) is crucial for saving millions of premature deaths every year due to tuberculosis. This paper describes a web portal developed for assisting researchers in designing vaccines against emerging Mtb strains using traditional and modern approaches. Firstly, we annotated 59 genomes of Mycobacterium species to understand similarity/dissimilarity between tuberculoid, non-tuberculoid and vaccine strains at genome level. Secondly, antigen-based vaccine candidates have been predicted in each Mtb strain. Thirdly, epitopes-based vaccine candidates were predicted/discovered in above antigen-based vaccine candidates that can stimulate all arms of immune system. Finally, a database of predicted vaccine candidates at epitopes as well at antigen level has been developed for above strains. In order to design vaccine against a newly sequenced genome of Mtb strain, server integrates three modules for identification of strain-, antigen-, epitope-specific vaccine candidates. We observed that 103522 unique peptides (9mers) had the potential to induce an antibody response and/or promiscuous binder to MHC alleles and/or have the capability to stimulate T lymphocytes. In summary, this web-portal will be useful for researchers working on designing vaccines against Mtb including drug-resistant strains. Availability: The database is available freely at http://crdd.osdd.net/raghava/mtbveb/.