Type III collagen is essential for growth acceleration of human osteoblastic cells by ascorbic acid 2-phosphate, a long-acting vitamin C derivative
Type III collagen is essential for growth acceleration of human osteoblastic cells by ascorbic acid 2-phosphate, a long-acting vitamin C derivative
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DOI：
10.1016/j.matbio.2007.01.005
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发表时间：
2007-06-01
期刊：
影响因子：
6.9
通讯作者：
Hata, Ryu-Ichiro
中科院分区：
文献类型：
--
作者：
Maehata, Yojiro;Takamizawa, Shinji;Hata, Ryu-Ichiro
据报道，胶原蛋白对于包括人成骨细胞在内的各种细胞的增殖是必需的[Takamizawa，S.，Maehata，Y.，今井，K.，Senoo，H.，Sato，S.，Hata河，2004.抗坏血酸和长效维生素C衍生物抗坏血酸2-磷酸盐对人成骨样细胞增殖和分化的影响。Cell Biol.Int.28，255-265]，但是负责生长调节的胶原的类型是未知的。目前，我们发现，抗坏血酸2-磷酸，一种长效维生素C衍生物，刺激细胞生长和人成骨细胞样MG-63细胞和正常人成骨细胞中III型胶原mRNA的表达，以及在人骨髓间充质干细胞中，但不是I型胶原在这些细胞中的表达。表皮生长因子还刺激MG-63细胞中的细胞生长和III型胶原mRNA的表达。在MG-63细胞克隆中，它们的生长速率与它们的COL 3A 1信使RNA水平显著相关，但与它们的COL 1A 1或COL 1A 2信使RNA水平无关。用针对COL 3A 1的siRNA转染MG-63细胞，而不转染针对COL 1A 1的siRNA，转染细胞的生长速率降低，同时COL 3A 1 mRNA水平下降。此外，当MG-63细胞在III型胶原包被的培养皿上培养时，通过胸苷掺入DNA和细胞数量观察到的细胞增殖增加。两者合计，我们的研究结果表明，III型胶原是负责人成骨细胞的生长刺激的胶原成分。(c)2007年Elsevier B. V./国际矩阵生物学学会。All rights reserved.
Collagen has been reported to be essential for the proliferation of various kinds of cells including human osteoblastic cells [Takamizawa, S., Maehata, Y., Imai, K., Senoo, H., Sato, S., Hata, R., 2004. Effects of ascorbic acid and ascorbic acid 2-phosphate, a long-acting vitamin C derivative, on the proliferation and differentiation of human osteoblast-like cells. Cell Biol. Int. 28, 255-265], but the type(s) of collagen responsible for growth regulation is not known. Presently we found that ascorbic acid 2-phosphate, a long-acting vitamin C derivative, stimulated both cell growth and the expression of mRNA for type III collagen in human osteoblast-like MG-63 cells and in normal human osteoblasts, as well as in human bone marrow mesenchymal stem cells, but not the expression of type I collagen in these cells. Epidermal growth factor also stimulated both cell growth and expression of type III collagen mRNA in MG-63 cells. Among MG-63 cell clones, their growth rates correlated significantly with their COL3A1 messenger RNA levels but not with their COL1A1 or COL1A2 messenger RNA levels. Transfection of MG-63 cells with siRNA for COL3A1 but not with that for COL1A1 decreased the growth rates of the transfected cells concomitant with a drop in the level of COL3A1 mRNA. Furthermore, cell proliferation as observed by thymidine incorporation into DNA and cell number was increased when MG-63 cells were cultured on type III collagen-coated dishes. Taken together, our results indicate that type III collagen is the collagen component responsible for the growth stimulation of human osteoblastic cells. (c) 2007 Elsevier B.V./International Society of Matrix Biology. All rights reserved.