Simple, Rapid, and Large‐Scale Fabrication of Multi‐Branched Hydrogels Based on Viscous Fingering for Cell Culture Applications
Simple, Rapid, and Large‐Scale Fabrication of Multi‐Branched Hydrogels Based on Viscous Fingering for Cell Culture Applications
复制标题
基于粘性指法的多分支水凝胶的简单、快速、大规模制造，用于细胞培养应用
DOI：
10.1002/mabi.202300069
复制
发表时间：
2023
影响因子：
4.6
通讯作者：
Shiku Hitoshi
中科院分区：
文献类型：
--
作者：
Utagawa Yoshinobu;Ino Kosuke;Hiramoto Kaoru;Shiku Hitoshi
水凝胶广泛用于细胞培养应用。为了制造组织和器官，必须生产具有特定结构的水凝胶。例如，多分支水凝胶对于开发类似于生物血管网络的网络结构是理想的。然而，现有技术对于该应用来说效率低且耗时。为了解决这个问题，提出了一种基于粘性指法的简单、快速、大规模的制造方法。该方法仅使用两个板。为了产生稀溶液，将高粘度溶液引入板之间的空间，并将其中一个板剥离。在此过程中，溶液的高粘度导致多支链结构的形成。使用这种策略，180 mm × 200 mm 多支化 Pluronic F-127 水凝胶在 1 分钟内成功制备。这些结构用作制造聚二甲基硅氧烷通道的牺牲层，用于培养人脐静脉内皮细胞（HUVEC）。类似地，制备了多支化基质胶和海藻酸钙（Ca）-海藻酸水凝胶结构，并在水凝胶内成功培养了 HUVEC。此外，从板上收集水凝胶，同时保持其结构。所提出的制造技术将有助于网络架构的发展，例如组织工程中的血管结构。
Hydrogels are widely used in cell culture applications. For fabricating tissues and organs, it is essential to produce hydrogels with specific structures. For instance, multiple‐branched hydrogels are desirable for the development of network architectures that resemble the biological vascular network. However, existing techniques are inefficient and time‐consuming for this application. To address this issue, a simple, rapid, and large‐scale fabrication method based on viscous fingering is proposed. This approach utilizes only two plates. To produce a thin solution, a high‐viscosity solution is introduced into the space between the plates, and one of the plates is peeled off. During this procedure, the solution's high viscosity results in the formation of multi‐branched structures. Using this strategy, 180 mm × 200 mm multi‐branched Pluronic F‐127 hydrogels are successfully fabricated within 1 min. These structures are used as sacrificial layers for the fabrication of polydimethylsiloxane channels for culturing human umbilical vein endothelial cells (HUVECs). Similarly, multi‐branched Matrigel and calcium (Ca)‐alginate hydrogel structures are fabricated, and HUVECs are successfully cultured inside the hydrogels. Also, the hydrogels are collected from the plate, while maintaining their structures. The proposed fabrication technique will contribute to the development of network architectures such as vascular structures in tissue engineering.