HLA B37 determines an influenza A virus nucleoprotein epitope recognized by cytotoxic T lymphocytes.
HLA B37 determines an influenza A virus nucleoprotein epitope recognized by cytotoxic T lymphocytes.
复制标题
DOI：
10.1084/jem.164.5.1397
复制
发表时间：
1986-11-01
期刊：
影响因子：
--
通讯作者：
Rothbard J
中科院分区：
文献类型：
--
作者：
McMichael AJ;Gotch FM;Rothbard J
人甲型流感病毒特异性细胞毒性T细胞先前已显示识别感染细胞上的病毒核蛋白。来自四个HLA B37阳性供体的CTL制剂显示识别对应于核蛋白序列的氨基酸335-349的合成肽。来自其他HLA类型的10个供体的流感特异性CTL未能识别该表位。CD 8 + CTL系来源于两个HLA B37阳性供体的淋巴细胞，并用于显示该肽在病毒感染的细胞上呈现，并确定表位的可能边界。
Human influenza A virus-specific, cytotoxic T cells have been shown previously to recognize the virus nucleoprotein on infected cells. CTL preparations from four HLA B37-positive donors were shown to recognize a synthetic peptide that corresponded to amino acids 335-349 of the nucleoprotein sequence. Influenza-specific CTL from 10 donors of other HLA types failed to recognize this epitope. CD8+ CTL lines were derived from lymphocytes of two HLA B37-positive donors and used to show that the peptide was represented on virus-infected cells and to determine the probable boundaries of the epitope.