Complex polyploid and hybrid species in an apomictic and sexual tropical forage grass group: genomic composition and evolution in Urochloa (Brachiaria) species.
Complex polyploid and hybrid species in an apomictic and sexual tropical forage grass group: genomic composition and evolution in Urochloa (Brachiaria) species.
复制标题
DOI：
10.1093/aob/mcab147
复制
发表时间：
2023-02-07
期刊：
影响因子：
4.2
通讯作者：
中科院分区：
文献类型：
--
作者：
二倍体和多倍体尾序草属（包括臂形草属、黍属和巨穗草属）C4热带牧草原产于非洲，对粮食安全和环境具有重要意义，通常种植在世界各地的边缘土地上。我们的目的是表征其基因组的性质，重复的DNA和多倍体的基因组组成，导致在包括许多无融合生殖物种的组内的进化途径的模型。对362份国际牧草种质资源进行了倍性分析，并采用流式细胞仪对倍性进行了测定。采用全基因组测序和分子细胞遗传学分析方法，对属于“brizantha”和“humidicola”无配子复合体的尾壳属和属于“humidicola”无配子复合体的尾壳属材料的染色体和基因组进行了鉴定。马克西姆基因组结构复杂多变，具有多种倍性和基因组组成的物种，并没有明确的地理模式。对9份二倍体和多倍体种质进行序列分析，发现了丰富的基因组特异性重复DNA基序。原位杂交与重复DNA和基因组DNA探针的组合确定了进化的分歧，使我们能够区分不同的基因组中存在的多倍体。我们建议一个新的连贯命名的基因组。我们开发了一个模型，在全基因组水平的二倍体和多倍体加入显示草的进化过程。我们支持保留窄种的概念，Uroplagabrizantha，U。decumbens和U. ruziziensis的细胞型，不认为单种的二倍体和多倍体是细胞型。研究结果和模型将有助于合理选择新杂交种的亲本，有助于利用种质资源进行育种和选择具有改善可持续性和农艺潜力的Urographa，并有助于测量和保护草原生物多样性。
Diploid and polyploid Urochloa (including Brachiaria, Panicum and Megathyrsus species) C4 tropical forage grasses originating from Africa are important for food security and the environment, often being planted in marginal lands worldwide. We aimed to characterize the nature of their genomes, the repetitive DNA and the genome composition of polyploids, leading to a model of the evolutionary pathways within the group including many apomictic species. Some 362 forage grass accessions from international germplasm collections were studied, and ploidy was determined using an optimized flow cytometry method. Whole-genome survey sequencing and molecular cytogenetic analysis were used to identify chromosomes and genomes in Urochloa accessions belonging to the ‘brizantha’ and ‘humidicola’ agamic complexes and U. maxima. Genome structures are complex and variable, with multiple ploidies and genome compositions within the species, and no clear geographical patterns. Sequence analysis of nine diploid and polyploid accessions enabled identification of abundant genome-specific repetitive DNA motifs. In situ hybridization with a combination of repetitive DNA and genomic DNA probes identified evolutionary divergence and allowed us to discriminate the different genomes present in polyploids. We suggest a new coherent nomenclature for the genomes present. We develop a model of evolution at the whole-genome level in diploid and polyploid accessions showing processes of grass evolution. We support the retention of narrow species concepts for Urochloa brizantha, U. decumbens and U. ruziziensis, and do not consider diploids and polyploids of single species as cytotypes. The results and model will be valuable in making rational choices of parents for new hybrids, assist in use of the germplasm for breeding and selection of Urochloa with improved sustainability and agronomic potential, and assist in measuring and conserving biodiversity in grasslands.
登录
查看更多内容
从矮牵牛亲本基因组洞察茄科的进化
影响因子：
18
作者：
Bombarely, Aureliano;Moser, Michel;Kuhlemeier, Cris
通讯作者：
Kuhlemeier, Cris
DOI：
10.1590/s1806-66902014000100008
发表时间：
2014-03-01
期刊：
Revista Ciência Agronômica
影响因子：
--
作者：
Timbó, Ana Luiza de Oliveira;Pereira, Roselaine Cristina;Davide, Lisete Chamma
通讯作者：
Davide, Lisete Chamma
影响因子：
2
作者：
de Souza-Kaneshima, A. M.;Simioni, C.;do Valle, C. B.
通讯作者：
do Valle, C. B.
影响因子：
11.6
作者：
Cheng, Feng;Mandakova, Terezie;Wang, Xiaowu
通讯作者：
Wang, Xiaowu
DOI：
10.1139/b87-313
发表时间：
1987-11-01
期刊：
CANADIAN JOURNAL OF BOTANY-REVUE CANADIENNE DE BOTANIQUE
影响因子：
--
作者：
BASAPPA, GP;MUNIYAMMA, M;CHINNAPPA, CC
通讯作者：
CHINNAPPA, CC