Determining auditory-evoked activities from multiple cells in layer 1 of the dorsal cortex of the inferior colliculus of mice by in vivo calcium imaging
Determining auditory-evoked activities from multiple cells in layer 1 of the dorsal cortex of the inferior colliculus of mice by in vivo calcium imaging
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DOI：
10.1016/j.brainres.2014.09.049
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发表时间：
2014-11-24
期刊：
影响因子：
2.9
通讯作者：
Ikeda, Hiroshi
中科院分区：
文献类型：
--
作者：
Ito, Tetsufumi;Hirose, Junichi;Ikeda, Hiroshi
下丘背皮层（DCIC）的第一层以其细胞结构和纤维连接区别于其他层。然而，由于在如此薄的结构上放置电极是具有挑战性的，因此DCIC第一层中所代表的声音类型的信息仍然不清楚。在本研究中，我们利用体内钙成像技术来评估DCIC第1层多个细胞的听觉诱发活动，并表征产生强烈活动的声音刺激。大多数被检查的细胞对宽带噪声和高声强的低频音调爆发表现出强烈的反应。在某些情况下，我们成功地获得了频率响应区域，这是音调频率和强度的接受域，其中30%的频率响应区域显示出v形调谐。这是第一个记录DCIC第一层细胞听觉反应的系统研究。这些结果表明，该区域的细胞对低频音调具有选择性，这暗示了这种听觉信息在DCIC第一层神经回路中的重要性。(C) 2014 Elsevier B.V.版权所有
Layer 1 of the dorsal cortex of the inferior colliculus (DCIC) is distinguished from other layers by its cytoarchitecture and fiber connections. However, the information of the sound types represented in layer 1 of the DCIC remains unclear because placing electrodes on such thin structures is challenging. In this study, we utilized in vivo calcium imaging to assess auditory-evoked activities in multiple cells in layer 1 of DCIC and to characterize sound stimuli producing strong activity. Most cells examined showed strong responses to broad-band noise and low-frequency tone bursts of high sound intensity. In some cases, we successfully obtained frequency response areas, which are receptive fields to tone frequencies and intensities, and similar to 30% of these showed V-shape tunings. This is the first systematic study to record auditory responses of cells in layer 1 of DCIC. These results indicate that cells in this area are selective to tones with low frequency, implying the importance of such auditory information in the neural circuitry of layer 1 of DCIC. (C) 2014 Elsevier B.V. All rights reserved.