A functional study of miR-124 in the developing neural tube
A functional study of miR-124 in the developing neural tube
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DOI：
10.1101/gad.1519207
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发表时间：
2007-03-01
影响因子：
10.5
通讯作者：
Gage, Fred H.
中科院分区：
文献类型：
--
作者：
Cao, Xinwei;Pfaff, Samuel L.;Gage, Fred H.
神经发育是一个高度协调的过程，需要精确控制基因表达。尽管microrna （mirna）与微调基因网络有关，但个体mirna在脊椎动物神经发育中的作用尚未在体内研究。我们研究了最丰富的神经元miRNA miR-124在脊髓发育过程中的功能。抑制或过表达miR-124都不会显著改变神经元命运的获得，这表明miR-124不太可能作为神经元分化的主要决定因素。miR-124的两个内源性靶点，层粘胶蛋白γ 1和整合素β 1，被鉴定出来，两者都在神经祖细胞中高表达，但在神经元分化中受到抑制。因此，miR-124似乎确保祖基因在神经元中被转录后抑制。
Neural development is a highly orchestrated process that entails precise control of gene expression. Although microRNAs (miRNAs) have been implicated in fine-tuning gene networks, the roles of individual miRNAs in vertebrate neural development have not been studied in vivo. We investigated the function of the most abundant neuronal miRNA, miR-124, during spinal cord development. Neither inhibition nor overexpression of miR-124 significantly altered the acquisition of neuronal fate, suggesting that miR-124 is unlikely to act as a primary determinant of neuronal differentiation. Two endogenous targets of miR-124, laminin gamma 1 and integrin beta 1, were identified, both of which are highly expressed by neural progenitors but repressed upon neuronal differentiation. Thus miR-124 appears to ensure that progenitor genes are post-transcriptionally inhibited in neurons.