In vitro analysis of a hepatic device with intrinsic microvascular-based channels
In vitro analysis of a hepatic device with intrinsic microvascular-based channels
复制标题
DOI：
10.1007/s10544-008-9194-3
复制
发表时间：
2008-12-01
影响因子：
2.8
通讯作者：
Neville, Craig
中科院分区：
文献类型：
--
作者：
Carraro, Amedeo;Hsu, Wen-Ming;Neville, Craig
描述了一种新型的基于微流体的双层装置，其具有模仿肝脏器官结构的离散实质室。使用计算模型设计微流体网络，以基于来自人类微脉管系统的生理数据提供适当的流动行为。图案化的硅晶片模具用于通过软光刻产生具有基于血管的微流体网络设计和实质腔室的膜。组装好的装置将肝细胞藏在纳米多孔膜后面，该纳米多孔膜允许代谢物和小蛋白质的运输，同时保护它们免受剪切应力的影响。该装置可以通过连续体外灌注培养基维持人肝癌细胞和原代大鼠肝细胞，允许增殖并维持肝脏功能，如血清蛋白合成和代谢。设计和制造过程是可扩展的，使设备概念既可以作为药物发现和毒性的平台技术，也可以用于改进肝脏辅助设备的持续开发。
A novel microfluidics-based bilayer device with a discrete parenchymal chamber modeled upon hepatic organ architecture is described. The microfluidics network was designed using computational models to provide appropriate flow behavior based on physiological data from human microvasculature. Patterned silicon wafer molds were used to generate films with the vascular-based microfluidics network design and parenchymal chamber by soft lithography. The assembled device harbors hepatocytes behind a nanoporous membrane that permits transport of metabolites and small proteins while protecting them from the effects of shear stress. The device can sustain both human hepatoma cells and primary rat hepatocytes by continuous in vitro perfusion of medium, allowing proliferation and maintaining hepatic functions such as serum protein synthesis and metabolism. The design and fabrication processes are scalable, enabling the device concept to serve as both a platform technology for drug discovery and toxicity, and for the continuing development of an improved liver-assist device.