Toxin profiles of Vibrio cholerae non-O1 from environmental sources in Calcutta, India
Toxin profiles of Vibrio cholerae non-O1 from environmental sources in Calcutta, India
复制标题
印度加尔各答环境来源的非 O1 霍乱弧菌毒素概况
DOI：
--
复制
发表时间：
1988
影响因子：
4.4
通讯作者：
Tae Takeda
中科院分区：
文献类型：
--
作者：
G. Nair;Y. Oku;Y. Takeda;A. Ghosh;R. K. Ghosh;S. Chattopadhyay;Pal Sc;J. B. Kaper;Tae Takeda
收集霍乱弧菌非O1从加尔各答，霍乱高流行区的水生环境中分离，霍乱毒素（CT），志贺样毒素（Vero毒素），热稳定性肠毒素和溶血素的生产进行了检查。2株（0.5%）非O1型霍乱弧菌分离株产生CT。来自这两个分离物的DNA也与含有编码CT的A亚基的序列的DNA探针杂交。没有菌株产生志贺样毒素或热稳定肠毒素。在89.7%的菌株中观察到溶血活性，其中36.1%在乳鼠中表现出生物活性。然而，他们都没有产生溶血素，交叉反应的热稳定性直接溶血弧菌。从这项研究中可以看出，一小部分环境中的非O1霍乱弧菌菌株确实具有引起霍乱样腹泻的潜力。
A collection of Vibrio cholerae non-O1 isolated from the aquatic environs of Calcutta, a cholera-hyperendemic area, were examined for the production of cholera toxin (CT), Shiga-like toxins (Vero toxins), heat-stable enterotoxin, and hemolysins. Two (0.5%) V. cholerae non-O1 isolates produced CT. The DNA from both these isolates also hybridized with a DNA probe containing sequences encoding the A subunit of CT. None of the strains produced Shiga-like toxins or heat-stable enterotoxin. Hemolytic activity was observed in 89.7% of the strains, of which 36.1% exhibited biological activity in the suckling mouse. However, none of them produced a hemolysin that cross-reacted with the thermostable direct hemolysin of Vibrio parahaemolyticus. It appears from this study that a small percentage of environmental V. cholerae non-O1 strains do possess the potential for causing cholera-like diarrhea.