Erythrocyte membrane microviscosity and phospholipid composition in lead workers.
Erythrocyte membrane microviscosity and phospholipid composition in lead workers.
复制标题
铅工红细胞膜微粘度及磷脂组成。
DOI：
10.1136/oem.44.12.841
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Maxell,RC
中科院分区：
文献类型：
--
作者：
Cook,LR;Stohs,SJ;Angle,CR;Hickman,TI;Maxell,RC
使用荧光团 1,6-二苯基-1,3,5-己三烯 (DPH) 通过荧光偏振测量来自主导工作人员和对照受试者的红细胞鬼膜的微粘度和流动性。铅的增加与重新密封和未密封的红细胞膜的平均微粘度的显着降低有关。由于 DPH 荧光反映了膜中央的脂质组织，因此研究了磷脂代谢的两个方面。从红细胞血影膜中提取磷脂，并通过高效液相色谱法进行鉴定。磷脂酰胆碱与磷脂酰乙醇胺的比率（膜流动性的既定相关因素）在领导工人中显着增加。这是由于已知的铅工人红细胞胆固醇增加以及磷脂酰乙醇胺和胆固醇的结构不相容性，导致磷脂酰胆碱代偿性增加。来自对照受试者的红细胞鬼膜被磷脂合成的中间体重新密封，该中间体随着铅抑制红细胞嘧啶5'-核苷酸酶的减少而增加。与三磷酸胞苷、三磷酸尿苷、二磷酸胞苷胆碱或二磷酸胞苷乙醇胺一起孵育不会改变膜流动性。红细胞膜双层疏水核心的脂质区域的微粘度和膜的磷脂组成的改变可能是导致红细胞暴露于铅时的临床和生化不稳定性的缺陷。
The microviscosity and fluidity of erythrocyte ghost membranes from lead workers and control subjects was measured by fluorescence polarisation using the fluorophore, 1,6-diphenyl-1,3,5-hexatriene (DPH). Increased lead was associated with a significant decrease in the average microviscosity of resealed and unsealed erythrocyte membranes. Since DPH fluorescence reflects the organisation of lipids in the central core of the membrane, two aspects of phospholipid metabolism were investigated. Phospholipids were extracted from red blood cell ghost membranes and identified by high performance liquid chromatography. The ratio of phosphatidyl choline to phosphatidyl ethanolamine, an established correlate of membrane fluidity, was significantly increased in lead workers. This is attributed to the known increases in red blood cell cholesterol in lead workers and the structural incompatibility of phosphatidyl ethanolamine and cholesterol, which result in a compensatory increase of phosphatidyl choline. Erythrocyte ghost membranes from control subjects were resealed with the intermediates in phospholipid synthesis that increase with a lead inhibited decrease in red blood cell pyrimidine 5'-nucleotidase. Membrane fluidity was not modified by incubation with cytidine triphosphate, uridine triphosphate, cytidine diphosphate choline, or cytidine diphosphate ethanolamine. Alterations in the microviscosity of the lipid regions of the hydrophobic core of the erythrocyte membrane bilayer and in the phospholipid composition of the membrane may be defects which contribute to the clinical and biochemical instability of the red blood cell on exposure to lead.