The mup-4 locus in Caenorhabditis elegans is essential for hypodermal integrity, organismal morphogenesis and embryonic body wall muscle position.
The mup-4 locus in Caenorhabditis elegans is essential for hypodermal integrity, organismal morphogenesis and embryonic body wall muscle position.
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秀丽隐杆线虫中的 mup-4 位点对于皮下完整性、生物体形态发生和胚胎体壁肌肉位置至关重要。
DOI：
10.1093/genetics/146.1.165
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发表时间：
1997
期刊：
影响因子：
3.3
通讯作者：
Bucher,EA
中科院分区：
文献类型：
--
作者：
Gatewood,BK;Bucher,EA
mup-4是秀丽隐杆线虫胚胎体壁肌细胞正确定位所必需的一组基因的成员。themup -4表型的表达是可变的，在胚胎发育阶段，当蠕虫从细胞球拉长到蠕虫形状时，会出现三种离散的阻滞表型（有机体形态发生）。代表两种表型类别的突变体在没有成功完成伸长的情况下停止。第三表型类的突变体在完成伸长后停止。在伸长后停止的突变体表现出严重的背侧和腹侧体壁肌肉细胞位置异常，以及由于肌肉细胞位置异常导致的特征性身体形状扭结（Mup表型）。值得注意的是，对mup-4突变体的遗传镶嵌分析表明，mup-4基因的功能在AB谱系中是必不可少的，AB谱系产生了大部分皮下组织（表皮），这是与肌肉相互作用的组织。与遗传镶嵌数据一致，表型特征显示突变体在皮下完整性和形态上存在缺陷。我们的分析支持这样的结论，即mup-4对皮下功能至关重要，这种功能对于机体形态发生和维持体壁肌肉位置是必要的。
mup-4is a member of a set of genes essential for correct embryonic body wall muscle cell positions inCaenorhabditis elegans.Themup-4phenotype is variably expressed and three discrete arrest phenotypes arise during the phase of embryonic development when the worm elongates from a ball of cells to its worm shape (organismal morphogenesis). Mutants representing two of the phenotypic classes arrest without successful completion of elongation. Mutants of the third phenotypic class arrest after completion of elongation. Mutants that arrest after elongation display profound dorsal and ventral body wallmuscle cellposition abnormalities and a characteristic kinked body shape (the Mup phenotype) dueto the muscle cell position abnormalities. Significantly, genetic mosaic analysis ofmup-4mutants demonstrates thatmup-4gene function is essential in the AB lineage, which generates most of the hypodermis (epidermis), a tissue with which muscle interacts. Consistent with the genetic mosaic data, phenotypic characterizations reveal that mutants have defects in hypodermal integrity and morphology. Our analyses support the conclusion thatmup-4is essential for hypodermal function and that this function is necessary for organismal morphogenesis and for the maintenance of body wall muscle position.