In-solution digestion of glycoproteins for glycopeptide-based mass analysis.
In-solution digestion of glycoproteins for glycopeptide-based mass analysis.
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用于基于糖肽的质量分析的糖蛋白溶液内消化。
DOI：
10.1007/978-1-62703-146-2_8
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Desaire,Heather
中科院分区：
文献类型：
--
作者：
Go,EdenP;Rebecchi,KathrynR;Desaire,Heather
糖肽由糖蛋白用特异性或非特异性蛋白酶的酶消化产生。无论糖蛋白到糖肽和肽的这种酶促转化是在溶液中还是在凝胶中完成，有效的消化方案都是基于质谱的实验工作流程中成功结果的关键组成部分之一。本章概述了一种优化的溶液内消化方案，用于制备基于糖肽的质量分析样品。
Glycopeptides are generated from the enzymatic digestion of glycoproteins with a specific or nonspecific protease. Whether this enzymatic conversion of glycoproteins into glycopeptides and peptides is done in-solution or in-gel, an efficient digestion protocol is one of the key components of a successful outcome in a mass spectrometry-based experimental workflow. This chapter outlines an optimized in-solution digestion protocol to prepare samples for glycopeptide-based mass analysis.