Microbial degradation of an aliphatic polyester with a high melting point, poly(tetramethylene succinate)
Microbial degradation of an aliphatic polyester with a high melting point, poly(tetramethylene succinate)
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高熔点脂肪族聚酯聚（丁二酸四亚甲基酯）的微生物降解
DOI：
10.1128/aem.61.5.1828-1832.1995
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发表时间：
1995
影响因子：
4.4
通讯作者：
H. Tanaka
中科院分区：
文献类型：
--
作者：
H. Pranamuda;Y. Tokiwa;H. Tanaka
研究了高熔点脂肪族聚酯聚丁二酸丁二醇酯（PTMS）的生物降解性能。生态学研究表明，与降解低熔点聚酯聚己内酯（PCL）的微生物相比，PTMS降解微生物在土壤环境中的分布非常有限。然而，在观察到形成透明带的土壤样品中，PTMS降解微生物占微生物总数的0.2%至6.0%，这非常接近于观察到的PCL降解微生物的百分比（0.8%至8.0%）。从在具有乳化PTMS的琼脂平板上形成明显透明区的菌落中分离出五个菌株。菌株HT-6对PTMS的降解活性最高。培养8天后，它吸收了约60%的PTMS粉末。当使用PTMS乳液时，观察到更高的降解率，并且超过90%的PTMS在6天内被同化。通过气相色谱法分析PTMS降解产物，发现在培养过程中积累了1，4-丁二醇、4-羟基正丁酸酯和琥珀酸。PTMS膜的降解分两步进行：破碎，然后在膜表面形成半球形孔。菌株HT-6还能够同化PCL和聚（β-羟基丁酸酯）（PHB）。该粗酶具有广泛的底物特异性，能够降解低分子量的PTMS，PCL，PHB，甚至高分子量的PTMS。
The biodegradability of poly(tetramethylene succinate) (PTMS), a synthetic aliphatic polyester with a high melting point, was evaluated. The ecological study showed that the distribution of PTMS-degrading microorganisms in soil environments was quite restricted compared with the distribution of microorganisms that degrade poly((epsilon)-caprolactone) (PCL), a polyester with a low melting point. However, in soil samples in which the formation of a clear zone was observed, PTMS-degrading microorganisms constituted 0.2 to 6.0% of the total number of microorganisms, which is very close to the percentage (0.8 to 8.0%) observed for PCL-degrading microorganisms. Five strains were isolated from colonies which formed distinct clear zones on agar plates with emulsified PTMS. In liquid cultures of the isolates with ground PTMS powder, strain HT-6, an actinomycete, showed the highest PTMS degrading activity. It assimilated about 60% of the ground PTMS powder after 8 days of cultivation. When a PTMS emulsion was used, a higher degradation rate was observed and more than 90% of the PTMS was assimilated in 6 days. PTMS degradation products were analyzed by gas chromatography, and it was found that 1,4-butanediol, 4-hydroxy n-butyrate, and succinic acid accumulated during cultivation. Degradation of PTMS film by the strain occurred in two steps: fragmentation and then the formation of hemispherical holes on the surface of the film. Strain HT-6 was also able to assimilate PCL and poly((beta)-hydroxybutyrate) (PHB). The crude enzyme showed a wide range of substrate specificity, being able to degrade low-molecular-weight PTMS, PCL, PHB, and even high-molecular-weight PTMS.