Involvement of Irreversible Vacuolar Membrane Fragmentation in the Lethality of Food Emulsifier Diglycerol Monolaurate against Budding Yeast.
Involvement of Irreversible Vacuolar Membrane Fragmentation in the Lethality of Food Emulsifier Diglycerol Monolaurate against Budding Yeast.
复制标题
不可逆的液泡膜破碎参与食品乳化剂单月桂酸甘油酯对芽殖酵母的杀伤力。
DOI：
10.1021/acs.jafc.7b01580
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Tanaka T.
中科院分区：
文献类型：
--
作者：
Ikegawa C;Ogita A;Doi T;Kumazawa F;Fujita KI;Tanaka T.
单月桂酸二甘油酯（DGL）是一种新型的食品乳化剂，正在研究作为食品防腐剂的进一步应用。DGL的致死率因此在酿酒酵母菌（一种引起食物腐败的酵母菌模型）中进行了检验。尽管其分子结构为非离子表面活性剂，但DGL在浓度低于对酵母质膜造成破坏性破坏的浓度时仍具有致死性。DGL的致死性往往伴随着一个动态的细胞内事件，如明显的空泡膜断裂。在dgl处理的细胞中，破碎的液泡膜的微小点或颗粒在脱离介质后未能融合到原来的大圆形结构中，这与在高渗条件下通常加速的液泡裂变所产生的结构不同。这种细胞器膜的不可逆结构损伤被认为是DGL致死性的原因。
Diglycerol monolaurate (DGL) has been manufactured as a novel type of food emulsifier and is being considered for further application as a food preservative. DGL lethality was thus examined againstSaccharomyces cerevisiaeas a model of a yeast that causes food spoilage. In spite of its molecular structure as a nonionic surfactant, DGL could exhibit lethality at a concentration lower than that which caused disruptive damage to the yeast plasma membrane. DGL lethality was rather accompanied by a dynamic intracellular event such as a marked vacuolar membrane fragmentation. In DGL-treated cells, the tiny dots or particles of fragmented vacuolar membranes failed to fuse into the original large rounded architecture after its removal from medium, which were distinguished from those generated as a result of vacuolar fission normally accelerated under hyperosmotic conditions. Such an irreversible structural damage of the organelle membrane was considered a cause of DGL lethality.