Identification and characterization of CONSTANS-like (COL) gene family in upland cotton (Gossypium hirsutum L.).
Identification and characterization of CONSTANS-like (COL) gene family in upland cotton (Gossypium hirsutum L.).
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陆地棉 (Gossypium hirsutum L.) 类 CONSTANS (COL) 基因家族的鉴定和表征
DOI：
10.1371/journal.pone.0179038
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Huang X
中科院分区：
文献类型：
--
作者：
Cai D;Liu H;Sang N;Huang X
CONSTANS/FLOWERLOCUS T（CO/FT）调节子在光周期敏感植物的开花时间控制中起核心作用。野生棉开花时间的研究具有严格的光周期敏感性，但驯化棉花是日中性的。关于棉花中CO和CO样（COL）基因的分子特征的信息非常有限。在本研究中，我们在G. hirsutum基因组中，其中许多是以前未报道的。我们研究了它们的染色体分布、系统发育关系以及基因和蛋白质的结构。结果表明，GhCOLs可分为3类，其中第1类中有14个COLs与其它植物相比具有保守的结构。第I组中的两个同源序列对GhCOL 1-A和GhCOL 1-D与拟南芥CO和水稻CO同源基因Heading date 1（Hd 1）的序列相似性最高。组织特异性表达表明，42 GhCOL基因可能在不同的细胞或器官中作为组织特异性调节剂发挥作用。我们克隆并测序了与开花诱导相关的第一组14个GhCOL基因，研究了它们的昼夜表达模式，发现它们的表达具有明显的昼夜节律调节。其中大多数在黎明时达到峰值，并在黄昏时迅速下降到最低值，然后开始积累，直到黎明后的长或短日照条件下。在拟南芥co-2突变体中的转基因研究表明，GhCOL 1-A和GhCOL 1-D完全拯救了晚花表型，而GhCOL 3-A、GhCOL 3-D、GhCOL 7-A和GhCOL 7-D部分拯救了晚花表型，而组I中的其他5个同源突变体对不促进开花。这些结果表明，GhCOL 1-A和GhCOL 1-D是棉花潜在的成花诱导基因，是棉花成花调控研究的候选基因。
The CONSTANS/FLOWERING LOCUS T (CO/FT) regulon plays a central role in the control of flowering time in photoperiod-sensitive plants. Flowering time in wild cotton (Gossypium spp.) has strict photoperiod sensitivity, but domesticated cotton is day-neutral. Information on the molecular characterization of the CO and CO-like (COL) genes in cotton is very limited. In this study, we identified 42 COL homologs (GhCOLs) in the G. hirsutum genome, and many of them were previously unreported. We studied their chromosome distribution, phylogenetic relationships, and structures of genes and proteins. Our results showed that GhCOLs were classified into three groups, and 14 COLs in group I showed conserved structure when compared with other plants. Two homoeologous pairs, GhCOL1-A and GhCOL1-D in Group I, showed the highest sequence similarity to Arabidopsis thaliana CO and rice CO homologous gene Heading date1 (Hd1). Tissue-specific expression showed that 42 GhCOL genes may function as tissue-specific regulators in different cells or organs. We cloned and sequenced the 14 GhCOL genes in Group I related to flowering induction to study their diurnal expression pattern, and found that their expression showed distinct circadian regulation. Most of them peaked at dawn and decreased rapidly to their minima at dusk, then started to accumulate until following dawn under long- or short-day conditions. Transgenic study in the Arabidopsis co-2 mutant demonstrated that GhCOL1-A and GhCOL1-D fully rescued the late-flowering phenotype, whereas GhCOL3-A, GhCOL3-D, GhCOL7-A, and GhCOL7-D partially rescued the late-flowering phenotype, and the other five homoeologous pairs in Group I did not promote flowering. These results indicate that GhCOL1-A and GhCOL1-D were potential flowering inducers, and are candidate genes for research in flowering regulation in cotton.