A 1H‐NMR method for determining temperature in cell culture perfusion systems
A 1H‐NMR method for determining temperature in cell culture perfusion systems
复制标题
确定细胞培养灌注系统温度的 1H-NMR 方法
DOI：
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复制
发表时间：
1993
影响因子：
3.3
通讯作者：
W. Hull
中科院分区：
文献类型：
--
作者：
N. Lutz;A. Kuesel;W. Hull
This report describes a noninvasive 1H‐NMR method for measuring absolute temperatures (±0.2°C) in biological samples and, in particular, in cell culture perfusion systems, utilizing the linear temperature dependence of the water chemical shift relative to the temperature‐independent shift of one of the components of the biological medium, e.g., pyruvate, acetate, or lactate. The effects of flow on temperature can be monitored and appropriate adjustments of the temperature controller can be made.
