Evidence for a high mutation rate at rapidly evolving yeast centromeres.
Evidence for a high mutation rate at rapidly evolving yeast centromeres.
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DOI：
10.1186/1471-2148-11-211
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发表时间：
2011-07-18
影响因子：
3.4
通讯作者：
Bensasson D
中科院分区：
文献类型：
--
作者：
Bensasson D
尽管着丝粒在细胞分裂中的作用是必不可少的，但着丝粒在动物、植物和酵母中进化得很快。与植物和氨基酸的复杂着丝粒不同，蔗糖酵母的点着丝粒可以很容易地测序，以区分着丝粒快速进化的可能原因。利用34株酿酒酵母所有16个着丝粒的DNA序列和矛盾酵母的群体基因组数据，我发现这两个物种的着丝粒进化速度都是选择性不受限制的DNA的3倍。在多个酵母种群中看到的异常高水平的多态表明，着丝粒的快速进化并不是减数分裂驱动下预期的重复选择性扫描的结果。我进一步表明，在着丝粒内几乎没有交换或基因转换的证据，尽管有明确的证据表明在它们的附近发生了重组。最后，我证明着丝粒的突变谱与基因组其他地方的自发突变模式是一致的。这些结果表明，着丝粒的快速进化是酵母物种中的一种普遍现象。此外，这些结果表明，着丝粒的快速进化不是基因转换的突变效应造成的，而是突变率的普遍增加，可能是由于酵母和其他真核生物着丝粒上不寻常的染色质结构造成的。
Although their role in cell division is essential, centromeres evolve rapidly in animals, plants and yeasts. Unlike the complex centromeres of plants and aminals, the point centromeres of Saccharomcyes yeasts can be readily sequenced to distinguish amongst the possible explanations for fast centromere evolution. Using DNA sequences of all 16 centromeres from 34 strains of Saccharomyces cerevisiae and population genomic data from Saccharomyces paradoxus, I show that centromeres in both species evolve 3 times more rapidly even than selectively unconstrained DNA. Exceptionally high levels of polymorphism seen in multiple yeast populations suggest that rapid centromere evolution does not result from the repeated selective sweeps expected under meiotic drive. I further show that there is little evidence for crossing-over or gene conversion within centromeres, although there is clear evidence for recombination in their immediate vicinity. Finally I show that the mutation spectrum at centromeres is consistent with the pattern of spontaneous mutation elsewhere in the genome. These results indicate that rapid centromere evolution is a common phenomenon in yeast species. Furthermore, these results suggest that rapid centromere evolution does not result from the mutagenic effect of gene conversion, but from a generalised increase in the mutation rate, perhaps arising from the unusual chromatin structure at centromeres in yeast and other eukaryotes.
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影响因子：
64.8
作者：
Kellis, M;Patterson, N;Lander, ES
通讯作者：
Lander, ES
影响因子：
64.8
作者：
Mancera, Eugenio;Bourgon, Richard;Brozzi, Alessandro;Huber, Wolfgang;Steinmetz, Lars M.
通讯作者：
Steinmetz, Lars M.
DOI：
10.1073/pnas.0803466105
发表时间：
2008-07-08
影响因子：
11.1
作者：
Lynch, Michael;Sung, Way;Thomas, W. Kelley
通讯作者：
Thomas, W. Kelley
DOI：
10.1126/science.1191125
发表时间：
2010-07-02
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Hicks WM;Kim M;Haber JE
通讯作者：
Haber JE
影响因子：
7
作者：
Mahtani, MM;Willard, HF
通讯作者：
Willard, HF