Origin of Deep Methane Associated with a Unique Community of Microorganisms in an Organic- and Iodine-Rich Aquifer
Origin of Deep Methane Associated with a Unique Community of Microorganisms in an Organic- and Iodine-Rich Aquifer
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深层甲烷的起源与富含有机物和碘的含水层中独特的微生物群落有关
DOI：
10.1021/acsearthspacechem.0c00204
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发表时间：
2021
影响因子：
3.4
通讯作者：
Ohkouchi Naohiko
中科院分区：
文献类型：
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作者：
Urai Atsushi;Takano Yoshinori;Imachi Hiroyuki;Ishii Shun’ichi;Matsui Yohei;Ogawara Miyuki;Tasumi Eiji;Miyairi Yosuke;Ogawa Nanako O.;Yoshimura Toshihiro;Inagaki Fumio;Yokoyama Yusuke;Kawano Kenjiro;Murai Daisuke;Park Ho-Dong;Ohkouchi Naohiko
我们进行了辅酶因子430（F430）分析和放射性碳测量，以确定深层微生物，包括产甲烷古菌，并评估甲烷的起源在一个深有机和碘丰富的含水层弧前盆地在南部关东气田，房总半岛，日本。在不存在F430差向异构体的情况下，我们检测到高浓度的天然F430（约1.67 × 104 femto mol L-1）。脂质和小亚基rRNA基因分析均表明，结构域古菌的生物量不到总原核生物的10%，而产甲烷古菌（例如，在rRNA序列中检测到甲烷微生物）。这些结果强烈表明地下微生物介导的高活性产甲烷潜力。甲烷的碳同位素比值稳定（δ 13 C甲烷，−67.9 ‰至−71.4‰，相对于维也纳皮迪贝莱姆岩），且富含高纯度甲烷（>99%），显示出典型的生物成因。甲烷和溶解无机碳（DIC）的放射性碳测量显示14 C-贫化（Δ 14 C甲烷和Δ 14 CDIC均<-997.4 ‰），这表明整个深层栖息地和甲烷生成代表了一个孤立的地下微生物生态系统。
We performed coenzyme factor 430 (F430) analysis and radiocarbon measurements to identify deep microorganisms, including methanogenic archaea, and assess the origin of methane in a deep organic- and iodine-rich aquifer in a forearc basin at the Southern Kanto gas field, Boso Peninsula, Japan. We detected high concentrations of native F430 (approximately 1.67 × 104femto mol L–1) in the absence of the F430 epimer. Both lipid and small-subunit rRNA gene analyses indicated that the biomass of domain archaea was less than 10% of the total prokaryotes, while methanogenic archaea (e.g.,Methanomicrobia) was detected in the rRNA sequences. These results strongly suggest high active methanogenesis potential mediated by the subsurface microbes. A stable carbon isotope ratio of methane (δ13Cmethane, −67.9 to −71.4‰ vs Vienna Pee Dee Belemnite) and a huge reservoir of high-purity methane (>99%) indicated a typical biogenic origin. Radiocarbon measurements of methane and dissolved inorganic carbon (DIC) revealed14C-depleted (both Δ14Cmethaneand Δ14CDIC, <−997.4‰), suggesting that the entire deep habitat and methanogenesis represents an isolated subterranean microbial ecosystem.