Characterization of TFG in Mus musculus and Caenorhabditis elegans
Characterization of TFG in Mus musculus and Caenorhabditis elegans
复制标题
DOI：
10.1006/bbrc.1999.0417
复制
发表时间：
1999-04-02
影响因子：
3.1
通讯作者：
Aman, P
中科院分区：
文献类型：
--
作者：
Mencinger, M;Aman, P
在人甲状腺乳头状癌中发现 TFG 作为 NTRK1 的融合伴侣。我们从 EST 序列和 5' 端 RACE 产物组装了小鼠 TFG cDNA，鉴定了猪 (c17b07) 和曼氏血吸虫 (SMNAS62) 中的全编码长度 TFG EST 克隆，并分析了秀丽隐杆线虫 (Y63D3A) 中 TFG 的基因组结构。小鼠、猪和曼氏沙门氏菌TFG的蛋白质序列与人类TFG高度同源。线虫序列已经出现分歧，但其预测的二级结构却非常保守。人类、小鼠和线虫 TFG 含有假定的三聚体 N 端卷曲螺旋结构域、糖基化、肉豆蔻酰化和磷酸化位点，以及 SH2 和 SH3 结合基序。线虫 TFG 中不存在 SH2 结合基序。 TFG 的表达在 7、11、15 和 19 天的小鼠胚胎阶段没有变化。在 10 个、5 天的全小鼠胚胎中使用 TFG 探针进行 Pn 原位杂交，显示出肢芽、鳃弓、鼻突和大脑的优先染色，以及原始脊髓和背根神经节的弱染色。 (C) 1999 年学术出版社。
TFG was discovered as a fusion partner of NTRK1 in human papillary thyroid carcinoma. We assembled the mouse TFG cDNA from EST sequences and 5' end RACE product, identified full coding length TFG EST clones in pig (c17b07) and Schistosoma mansoni (SMNAS62), and analyzed the genomic structure of TFG in Caenorhabditis elegans (Y63D3A). The protein sequences of mouse, pig, and S. mansoni TFG are highly homologous to human TFG. The C. elegans sequence has diverged, but its predicted secondary structure is remarkably conserved. Human, mouse, and C. elegans TFG contain a putative trimeric N-terminal coiled-coil domain, glycosylation, myristylation, and phosphorylation sites, and SH2- and SH3-binding motifs. The SH2-binding motif is absent in C. elegans TFG. The expression of TFG does not vary among 7, 11, 15, and 19 day mouse embryonal stages. Pn situ hybridization with a TFG probe in 10, 5-day whole mouse embryos showed preferential staining of the limb buds, branchial arches, nasal processes, and brain, and weak staining of the primitive spinal cord and dorsal root ganglia. (C) 1999 Academic Press.