A HYPOTHALAMIC ACTIVATOR OF CALMODULIN-DEPENDENT ENZYMES IS THYMOSIN-BETA-4 (1-39)
A HYPOTHALAMIC ACTIVATOR OF CALMODULIN-DEPENDENT ENZYMES IS THYMOSIN-BETA-4 (1-39)
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DOI：
10.1007/bf00969011
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发表时间：
1992-08-01
影响因子：
4.4
通讯作者：
LEE, TD
中科院分区：
文献类型：
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作者：
GALOYAN, AA;GURVITIS, BY;LEE, TD
一类新的刺激剂的基础活动的一些钙调素依赖性酶已被分离出牛下丘脑。这些刺激物之一，表示为C3，已通过反相HPLC纯化至均一，并通过质谱和Edman微序列分析初步鉴定为胸腺素β-4（1 - 39）。将C3对兔骨骼肌MLCK基础活性的刺激作用与胸腺素α-1和胸腺素β-4的刺激作用进行了比较（16 - 38）。有证据表明，所有指示的化合物都是钙离子非依赖性高亲和力MLCK刺激剂。激活酶的刺激剂的效力为：C3> β-4>（CaM + Ca 2+）> α-1。
A new class of stimulators of basal activity of a number of calmodulin-dependent enzymes have been previously isolated from bovine hypothalamus. One of these stimulators, denoted as C3, has been purified to homogeneity by reverse phase HPLC and tentatively identified as thymosin beta-4 (1 - 39) by mass spectrometry and Edman microsequence analysis. The stimulating effect of C3 on rabbit skeletal muscle MLCK basal activity was compared with that of thymosin alpha-1 and thymosin beta-4, (16 - 38). Evidence is presented that all the indicated compounds are Ca2+ -independent high-affinity MLCK stimulators. The potency of the stimulators in activating the enzyme was: C3> beta-4> (CaM + Ca2+)>alpha-1.