Fluoro-Jade B: a high affinity fluorescent marker for the localization of neuronal degeneration
Fluoro-Jade B: a high affinity fluorescent marker for the localization of neuronal degeneration
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DOI：
10.1016/s0006-8993(00)02513-0
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发表时间：
2000-08-25
期刊：
影响因子：
2.9
通讯作者：
Hopkins, KJ
中科院分区：
文献类型：
--
作者：
Schmued, LC;Hopkins, KJ
F-Jade B和它的前身F-Jade一样，是一种阴离子荧光素衍生物，用于变性神经元的组织学染色。然而，Fluoro-Jade B对变性神经元具有更高的特异性亲和力。这一观点得到了退化神经元成分的明显染色的支持，背景染色最少。这种改进的信噪比意味着包括远端树突、轴突和轴突终末在内的精细神经元突起可以更容易地被检测和记录下来。虽然减少了染色时间和染料浓度，但该方法与原来的荧光玉技术一样快速、简单和可靠。与Fluoro-Jade类似，Fluoro-Jade B与许多其他标签程序兼容，包括免疫荧光和荧光Nissl技术，(C)2000 Elsevier Science B.V.保留所有权利。
Fluoro-Jade B, like its predecessor Fluoro-Jade, is an anionic fluorescein derivative useful for the histological staining of neurons undergoing degeneration. However, Fluoro-Jade B has an even greater specific affinity for degenerating neurons. This notion is supported by the conspicuous staining of degenerating neuronal elements with minimal background staining. This improved signal-to-noise ratio means that fine neuronal processes including distal dendrites, axons and axon terminals can be more readily detected and documented. Although the staining time and dye concentration are reduced, the method is as rapid, simple and reliable as the original Fluoro-Jade technique. Like Fluoro-Jade, Fluoro-Jade B is compatible with a number of other labeling procedures including immunofluorescent and fluorescent Nissl techniques, (C) 2000 Elsevier Science B.V. All rights reserved.