Retromer and the dynamin Vps1 cooperate in the retrieval of transmembrane proteins from vacuoles
Retromer and the dynamin Vps1 cooperate in the retrieval of transmembrane proteins from vacuoles
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DOI：
10.1242/jcs.132720
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发表时间：
2015-02-15
影响因子：
4
通讯作者：
Ungermann, Christian
中科院分区：
文献类型：
--
作者：
Arlt, Henning;Reggiori, Fulvio;Ungermann, Christian
内体是动态细胞器，需要结合成功地将蛋白质和脂类运送到溶酶体样液泡，并将其他蛋白质和脂类循环到高尔基体或质膜的能力。我们现在证明，逆转聚体可以作用于液泡，它与蛋白质从内体到高尔基体或质膜的恢复有关。我们使用一种分析方法来探索它的功能，这种方法允许我们在回收过程中剖析所需的辅因子。我们证明，从液泡中回收跨膜受体Vps10需要逆聚体、动力素样的Vps1和Rab7GTPase Ypt7。逆转录酶和Vps1与货物一起离开液泡，而Ypt7留在后面，这与其调节功能一致。回收的货物随后在内体积累，然后在高尔基堆积，这意味着连续的分拣步骤到达最终目的地。我们的数据进一步表明，逆转聚体和Vps1对于维持液泡膜的组织是必不可少的。综上所述，我们的数据表明，逆转录可以与Vps1和Rab Ypt7合作，清除选定的膜蛋白的液泡。
Endosomes are dynamic organelles that need to combine the ability to successfully deliver proteins and lipids to the lysosome-like vacuole, and recycle others to the Golgi or the plasma membrane. We now show that retromer, which is implicated in retrieval of proteins from endosomes to the Golgi or to the plasma membrane, can act on vacuoles. We explore its function using an assay that allows us to dissect the required cofactors during recycling. We demonstrate that recycling of the transmembrane receptor Vps10 from vacuoles requires the retromer, the dynamin-like Vps1, and the Rab7 GTPase Ypt7. Retromer and Vps1 leave the vacuole together with the cargo, whereas Ypt7 stays behind, in agreement with its regulatory function. Recycled cargo then accumulates at endosomes and later at the Golgi, implying consecutive sorting steps to the final destination. Our data further suggest that retromer and Vps1 are essential to maintain vacuole membrane organization. Taken together, our data demonstrate that retromer can cooperate with Vps1 and the Rab Ypt7 to clear the vacuole of selected membrane proteins.