Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale.
Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale.
复制标题
在基因组规模上对 5-甲酰胞嘧啶进行无亚硫酸氢盐碱基分辨率分析。
DOI：
10.1038/nmeth.3569
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发表时间：
2015-11
期刊：
影响因子：
48
通讯作者：
Yi C
中科院分区：
文献类型：
--
作者：
Xia B;Han D;Lu X;Sun Z;Zhou A;Yin Q;Zeng H;Liu M;Jiang X;Xie W;He C;Yi C
哺乳动物活性DNA去甲基化涉及tet介导的5-甲基胞嘧啶（5mC）氧化为5-羟甲基胞嘧啶（5hmC）、5-甲酰胞嘧啶（5fC）和5-羧胞嘧啶（5caC）。然而，单碱基分辨率的5fC全基因组检测仍然具有挑战性。在这里，我们提出了一种基于5fC的选择性化学标记和PCR过程中随后的C-to-T转换的无亚硫酸盐全基因组分析方法。基础分辨率5fC地图显示与5hmC的重叠有限，5fC标记的区域比5hmC标记的区域更活跃。
Active DNA demethylation in mammals involves TET-mediated oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxycytosine (5caC). However, genome-wide detection of 5fC at single-base resolution remains challenging. Here we present a bisulfite-free method for whole-genome analysis of 5fC, based on selective chemical labeling of 5fC and subsequent C-to-T transition during PCR. Base-resolution 5fC maps reveal limited overlap with 5hmC, with 5fC-marked regions more active than 5hmC-marked ones.