Sequential pathology of genital tract in chickens experimentally infected with Mycoplasma gallisepticum.
Sequential pathology of genital tract in chickens experimentally infected with Mycoplasma gallisepticum.
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鸡毒支原体实验感染鸡生殖道的序贯病理学。
DOI：
10.2307/1590013
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发表时间：
1981
期刊：
影响因子：
1.4
通讯作者：
M. Kharole
中科院分区：
文献类型：
--
作者：
A. Pruthi;M. Kharole
从红霉素预处理蛋中孵化的未分性别的1日龄白色来航鸡通过卵黄囊感染致病性鸡毒支原体（S6）的48小时肉汤培养物。在57只实验雌禽中，有14只在输卵管中出现肉眼病变，其特征为在输卵管的一段或整个长度中存在各种大小的灰白色干酪样栓塞。46只受感染的雌禽有显微镜下病变，主要检测为轻度嗜异性浸润、淋巴细胞聚集体、淋巴滤泡、弥漫性淋巴细胞浸润、多细胞反应或任何这些的组合，取决于感染的阶段和程度。干酪栓由组织碎片、纤维蛋白、坏死的嗜异性粒细胞以及有时少量淋巴细胞和巨噬细胞形成。伴随坏死性渗出物的变化是粘膜萎缩，输卵管壁肥大，有时粘膜皱襞坏死。在卵巢中未检测到明显的肉眼和显微镜下病变，尽管33只感染的雄性鸡中有3只在睾丸中有组织学病变。在呼吸道中也发现了支原体病的大体和显微镜改变。M.在感染后5周内，鸡毒败血菌可持续从输卵管中再分离;此后，分离是间歇性的。从卵巢或睾丸中可持续分离出微生物，仅持续至PI 1周。感染后1周，鸡血清中就检测到凝集素，平均滴度为1：5，感染后6周达到最高，为1：128。此后，滴度逐渐下降，但可证实的凝集素存在，直到25周PI。
Unsexed day-old White Leghorn chicks hatched from eggs pre-treated with erythromycin were infected via the yolk sac with a 48-hr broth culture of pathogenic Mycoplasma gallisepticum (S6). Fourteen of the 57 experimental female birds had gross lesions in the oviduct, characterized by the presence of grayish-white caseous plugs of various sizes in a segment or throughout the length of the oviduct. Forty-six of the infected female birds had microscopic lesions, detected primarily as mild heterophilic infiltration, lymphocytic aggregates, lymphoid follicles, diffuse lymphocytic infiltration, pleocellular response, or a combination of any of these depending on the stage and extent of infection. The caseous plugs were formed by tissue debris, fibrin, necrotic heterophils, and sometimes a few lymphocytes and macrophages. Changes that accompanied the necrotic exudate were atrophy of the mucosa, hypertrophy of the oviduct wall, or sometimes necrosis of the folds of mucosa. No significant gross and microscopic lesions were detected in ovaries, although 3 of the 33 infected male chickens had histological lesions in the testes. Gross and microscopic changes of mycoplasmosis were also found in the respiratory tract. M. gallisepticum could be consistently reisolated from oviduct(s) up to five weeks PI; thereafter, isolations were intermittent. From the ovaries or testes the organism could be isolated consistently up to one week PI only. Agglutinins were detected in the sera of infected chickens as early as one week PI, when the mean titer was 1:5, and it reached a maximum of 1:128 at six weeks PI. Thereafter the titer gradually declined, but the demonstrable agglutinins were present until 25 weeks PI.