Coordinated expression of H3K9 histone methyltransferases during tooth development in mice
Coordinated expression of H3K9 histone methyltransferases during tooth development in mice
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DOI：
10.1007/s00418-014-1284-0
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发表时间：
2015-03-01
影响因子：
2.3
通讯作者：
Nifuji, Akira
中科院分区：
文献类型：
--
作者：
Kamiunten, Taichi;Ideno, Hisashi;Nifuji, Akira
组织特异性基因表达受表观遗传和遗传调控。组蛋白尾部的翻译后修饰改变了核蛋白对DNA的可及性，从而影响核蛋白调节复合物的活性。组蛋白3赖氨酸9（H3 K9）的甲基化是影响基因表达和细胞分化的关键修饰。已知H3 K9具有0-3个甲基化状态，并且这四个甲基化状态由组蛋白甲基转移酶组的表达决定。在发育过程中，牙齿是通过间充质和上皮之间的相互作用形成的，这一过程受到表观遗传调控。在这项研究中，我们研究了所有H3 K9甲基转移酶（H3 K9 MTases）在小鼠牙齿发育过程中的表达。我们发现四种H3 K9 MTases-G9 a，Glp，Prdm 2和Suv 39 h1-在牙胚中高度表达，在小鼠胚胎的16.5和17.5天左右表达高峰。免疫组织化学和原位杂交分析表明，所有四个H3 K9 MTases富集在间充质比在上皮。H3 K9 MTases、H3 K9 me 1、H3 K9 me 2和H3 K9 me 3的底物也在间充质中富集。综上所述，这些数据表明，协调表达的四个H3 K9 MTases在牙齿间充质中可能发挥重要作用。
Tissue-specific gene expression is subjected to epigenetic and genetic regulation. Posttranslational modifications of histone tails alter the accessibility of nuclear proteins to DNA, thus affecting the activity of the regulatory complex of nuclear proteins. Methylation at histone 3 lysine 9 (H3K9) is a crucial modification that affects gene expression and cell differentiation. H3K9 is known to have 0-3 methylation states, and these four methylated states are determined by the expression of sets of histone methyltransferases. During development, teeth are formed through mutual interactions between the mesenchyme and epithelium via a process that is subjected to the epigenetic regulation. In this study, we examined the expression of all H3K9 methyltransferases (H3K9MTases) during mouse tooth development. We found that four H3K9MTases-G9a, Glp, Prdm2, and Suv39h1-were highly expressed in the tooth germ, with expression peaks at around embryonic days 16.5 and 17.5 in mice. Immunohistochemical and in situ hybridization analyses revealed that all four H3K9MTases were enriched in the mesenchyme more than in the epithelium. Substrates of H3K9MTases, H3K9me1, H3K9me2, and H3K9me3 were also enriched in the mesenchyme. Taken together, these data suggested that coordinated expression of four H3K9MTases in the dental mesenchyme might play important roles in tooth development.