A marine oomycete Atkinsiella panulirata sp. nov. from philozoma of spiny lobster, Panulirus japonicus
A marine oomycete Atkinsiella panulirata sp. nov. from philozoma of spiny lobster, Panulirus japonicus
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DOI：
10.1007/bf02268578
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发表时间：
1995-01-01
期刊：
影响因子：
1.4
通讯作者：
Hatai, Kishio
中科院分区：
文献类型：
--
作者：
Kitancharoen, Nilubol;Hatai, Kishio
从多刺龙虾（Panulirus）的嗜水气单胞菌（philozoma）中分离到一种海洋卵菌。该真菌生长缓慢，偶尔浸没，具有乳白色白色凸起的潮湿菌落。菌丝粗壮，呈放射状排列，分枝不规则，直径10-22 μ m，偶被横壁分成亚叶状体。菌体偶尔会有肿胀的特征。孢子囊由亚体形成，在末端或亚末端有1-3条波状或部分卷曲的放电管。游动孢子梨形或肾形，双鞭毛，等粒体，双行。孢子在无菌人工海水中萌发为丝状体，顶端球形膨大，在PYGS肉汤中直接萌发为粗壮的初始菌丝。经鉴定，该菌属于Atkinsiella属，命名为Atkinsiella panulirata sp. nov.，最适生长温度为25 ℃。菌丝在1.5-6.0%的无菌人工海水和2.0- 4.0%的NaCl中均能生长。该菌能利用所有碳水化合物及其衍生物作为碳源。通过对5种杀菌剂的离体抑菌试验，发现孔雀石绿绿色的抑菌效果最好。
A marine oomycete fungus was isolated from philozoma of the spiny lobster, Panulirus japonicus. The fungus exhibited slow growth, occasionally submerged, with a creamy white, raised moist colony. Hyphae were stout, arranged in radiating pattern, irregularly branched, 10-22 mu-m diam, occasionally separated by cross walls into subthalli. Thalli occasionally consisted of swollen features. Sporangia formed from the subthalli had 1-3 wavy or partly coiled discharge tubes at the terminal or subterminal area. Zoospores were pyriform or reniform, biflagellate, isokont and diplanetic. Encysted spores germinated as a hairlike filament with a globular enlarged tip in sterilized synthetic seawater, and directly as stout initial hypha in PYGS broth. This fungus was identified as belonging to the genus Atkinsiella and was designated Atkinsiella panulirata sp. nov. The optimal temperature for the fungus was 25 degree C. Hyphal growth occurred in both sterilized synthetic seawater and NaCl in the range of 1.5-6.0% and 2.0-4.0%, respectively. The fungus could utilize all carbohydrates and derivatives tested as the carbon sources. From in vitro tests of five fungicides against the fungus malachite green was found to be the most effective.